Purification and Identification of 1-Deoxynojirimycin (DNJ) in Okara Fermented by Bacillus subtilis B2 from Chinese Traditional Food (Meitaoza)

ZHU, Yun-Ping ; YAMAKI, Kohji ; et al.

In: Journal of agricultural and food chemistry (Print), Jg. 58 (2010), Heft 7, S. 4097-4103

academicJournal - print, 29 ref

This study was to purify an α-glucosidase inhibitor from okara (soy pulp) fermented by Bacillus subtilis B2 and to identify its chemical structure. Membrane dialysis, active charcoal, CM-Sepharose chromatography, and preparative thin-layer chromatography (TLC) were used in the purification, while positive mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectrometry were used in the identification. The MS and NMR data showed that the purified α-glucosidase inhibitor was 1-deoxynojirimycin (DNJ) with a molecular weight of 163 Da. This is the first time that DNJ was isolated from foods fermented with Bacillus species. Okara fermentation with B. subtilis B2 might be used to produce a food-derived DNJ product as a functional food for diabetic patients.

Titel:	Purification and Identification of 1-Deoxynojirimycin (DNJ) in Okara Fermented by Bacillus subtilis B2 from Chinese Traditional Food (Meitaoza)
Autor/in / Beteiligte Person:	ZHU, Yun-Ping ; YAMAKI, Kohji ; YOSHIHASHI, Tadashi ; OHNISHI KAMEYAMA, Mayumi ; LI, Xiu-Ting ; CHENG, Yong-Qiang ; MORI, Yutaka ; LI, Li-Te
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Journal of agricultural and food chemistry (Print), Jg. 58 (2010), Heft 7, S. 4097-4103
Veröffentlichung:	Washington, DC: American Chemical Society, 2010
Medientyp:	academicJournal
Umfang:	print, 29 ref
ISSN:	0021-8561 (print)
Schlagwort:	Agronomy, agriculture, phytopathology Agronomie, agriculture, phytopathologie Food science technology Sciences technologies alimentaires Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Industries agroalimentaires Food industries Microbiologie alimentaire Food microbiology Alcaloïde Alkaloid Alcaloide Bacillaceae Bacillales Bactérie Bacteria Enzyme Enzima Glycosidases Glycosylases Hydrolases Aliment Food Alimento Bacillus subtilis Identification Identificación Inhibiteur Inhibitor Inhibidor Produit fermenté Fermented product Producto fermentado Purification Purificación α-Glucosidase 1-Désoxynojirimycine 1-deoxynojirimycin Bacillus subtilis B2 fermented okara purification α-Glucosidase inhibitor
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Department of Food Science, School of Chemical and Environmental Engineering, Beijing Technology and Business University, No. 33 Fucheng Road, Haidian District, Beijing 100048, China ; Post-harvest Science and Technology Division, Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki 305-8686, Japan ; National Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Ibaraki 305-8642, Japan ; College of Food Science and Nutritional Engineering, Post Box 40, China Agricultural University, East Campus, No. 17 Qinghua Dong Lu, Haidian District, Beijing 100083, China Rights: Copyright 2015 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Agrifood industries

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