A bienzyme electrode for L-malate based on a novel and general design
In: Journal of biotechnology, Jg. 61 (1998), Heft 2, S. 129-133
academicJournal
- print, 9 ref
Zugriff:
The coimmobilisation of a NAD(P)+ -dependent dehydrogenase with salicylate hydroxylase (SHL, EC 1.14.13.1) in front of a Clark-electrode yields a flexible new design for dehydrogenase based biosensors. The feasibility of the approach has been tested with malic enzyme (MDH, EC 1.1.1.40) as the dehydrogenase, resulting in a novel L-malate sensor. It had substantial advantages over the biosensor approaches reported earlier: effective re-oxidation of NADPH by SHL yielded an extended linear range from 0.01 to 1.2 mmol 1-1 L-malate and strongly reduced NADP+ -requirement ( < 0.025 mmol l-1 while the working stability was increased to more than 30 days. The results obtained from six real samples showed a close correlation with the standard enzymatic method. The presented scheme with SHL and the Clark-electrode can be employed together with any NAD(P)+-dependent dehydrogenase.
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A bienzyme electrode for L-malate based on a novel and general design
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Autor/in / Beteiligte Person: | GAJOVIC, N ; WARSINKE, A ; SCHELLER, F. W |
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Zeitschrift: | Journal of biotechnology, Jg. 61 (1998), Heft 2, S. 129-133 |
Veröffentlichung: | Amsterdam; Lausanne; New York, NY: Elsevier, 1998 |
Medientyp: | academicJournal |
Umfang: | print, 9 ref |
ISSN: | 0168-1656 (print) |
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