Ultra-fast two-dimensional microchip electrophoresis using SDS μ-CGE and microemulsion electrokinetic chromatography for protein separations

OSIRI, John K ; SHADPOUR, Hamed ; et al.

In: Analytical & bioanalytical chemistry (Print), Jg. 398 (2010), Heft 1, S. 489-498

Online academicJournal - print, 54 ref

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A poly(methyl methacrylate) microfluidic chip was used to perform a two-dimensional (2-D) separation of a complex protein mixture in short development times. The separation was performed by combining sodium dodecyl sulfate micro-capillary gel electrophoresis (SDS μ-CGE) with microemulsion electrokinetic chromatography (μ-MEEKC), which were used for the first and second dimensions, respectively. Fluorescently labeled Escherichia coli cytosolic proteins were profiled by this 2-D approach with the results compared to a similar 2-D separation using SDS μ-CGE × μ-MEKC (micelle electrokinetic chromatography). The relatively short column lengths (effective length=10 mm) for both dimensions were used to achieve separations requiring only 220 s of development time. High spot production rates (131 ± 11 spots min―1) and reasonable peak capacities (481 ± 18) were generated despite the fact that short columns were used. In addition, the use of μ-MEEKC in the second dimension was found to produce higher peak capacities compared to μ-MEKC (481 ± 18 for μ-MEEKC and 332 ± 17 for μ-MEKC) due to the higher plate numbers associated with μ-MEEKC.

Titel:	Ultra-fast two-dimensional microchip electrophoresis using SDS μ-CGE and microemulsion electrokinetic chromatography for protein separations
Autor/in / Beteiligte Person:	OSIRI, John K ; SHADPOUR, Hamed ; SOPER, Steven A
Link:	Volltext (PDF) View record from PASCAL Archive
Zeitschrift:	Analytical & bioanalytical chemistry (Print), Jg. 398 (2010), Heft 1, S. 489-498
Veröffentlichung:	Heidelberg: Springer, 2010
Medientyp:	academicJournal
Umfang:	print, 54 ref
ISSN:	1618-2642 (print)
Schlagwort:	Food science technology Sciences technologies alimentaires Analytical chemistry Chimie analytique Chemistry Chimie Sciences exactes et technologie Exact sciences and technology Méthodes chromatographiques et méthodes physiques associées à la chromatographie Chromatographic methods and physical methods associated with chromatography Autres méthodes chromatographiques Other chromatographic methods Bactérie Bacteria Enterobacteriaceae Mécanique fluide Fluid mechanics Mecánica flúido Chromatographie MECC Micellar electrokinetic capillary chromatography Cromatografía MECC Composé du sodium Sodium compound Sodio compuesto Electrocinétique Electrokinetics Electrocinética Electrophorèse bidimensionnelle Two dimensional electrophoresis Electrofóresis bidimensional Electrophorèse capillaire Capillary electrophoresis Electroforesis capilar Electrophorèse gel Gel electrophoresis Electroforesis gel Escherichia coli Micelle Micela Microfluidique Microfluidics Microfluidic Microémulsion Microemulsion Microemulsión Mélange Mixture Mezcla Méthacrylique acide ester méthyle Methyl methacrylate Metacrílico ácido ester metilo Pic Peak Pico Plaque Plate Placa Production Producción Protéine Protein Proteína Sulfate(lauryl) Lauryl sulfate Lauril sulfato Système sur puce System on a chip Sistema sobre pastilla Temps Time Tiempo Utilisation Use Uso E. coli proteins PMMA microchip Protein profiling SDS micro-CGE Two-dimensional electrophoresis micro-MEEKC
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, United States ; Center for BioModular Multi-Scale Systems, Louisiana State University, Baton Rouge, LA 70803, United States ; Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA 70803, United States Rights: Copyright 2015 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Analytical chemistry

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