The Development and Mechanism Studies of Cationic Chitosan-Modified Biodegradable PLGA Nanoparticies for Efficient siRNA Drug Delivery
In: Pharmaceutical research, Jg. 27 (2010), Heft 7, S. 1285-1295
Online
academicJournal
- print, 41 ref
Zugriff:
Purpose In order to improve siRNA delivery for possible clinical applications, we developed biodegradable chitosan-modified poly(D,L-lactide-co-glycolide) (CHT-PLGA) nanoparticles with positive surface charge, high siRNA loading, high transfection effciency and low toxicity. Methods CHT-PLGA nanoparticles were prepared, and siRNA was loaded by emulsion evaporation method with poly(vinyl alcohol) (PVA) as emulsifer. siRNA loading effciency, particle size, and Zeta potential of nanoparticles were measured. Gel retardation and protection assays were conducted to determine the loading and binding of siRNA in the formulation. Cell transfection was performed to study in vitro siRNA silencing efficiency. XTT assay was used to evaluate the cytotoxicity. Results It was found that the nanoparticle diameter and positive Zeta potential increase as the chitosan coating concentration increases. CHT-PLGA nanoparticles showed excellent siRNA binding ability and effective protection of oligos from RNase degradation. siRNA-loaded nanoparticles were successfully delivered into the HEK 293 T cell line, and the silencing of green fluorescence protein (GFP) expression was observed using fluorescent microscopy and flow cytometry. In addition, the cytotoxicity assay revealed that CHT-PLGA nanoparticles had relatively low cytotoxicity. Conclusion This study suggests that biodegradable cationic CHT-PLGA nanoparticles possess great potential for efficient and safer siRNA delivery in future clinical applications.
Titel: |
The Development and Mechanism Studies of Cationic Chitosan-Modified Biodegradable PLGA Nanoparticies for Efficient siRNA Drug Delivery
|
---|---|
Autor/in / Beteiligte Person: | XUDONG, YUAN ; SHAH, Bruhal A ; KOTADIA, Naimesh K ; JIAN, LI ; HUA, GU ; ZHIQIAN, WU |
Link: | |
Zeitschrift: | Pharmaceutical research, Jg. 27 (2010), Heft 7, S. 1285-1295 |
Veröffentlichung: | New York, NY: Springer, 2010 |
Medientyp: | academicJournal |
Umfang: | print, 41 ref |
ISSN: | 0724-8741 (print) |
Schlagwort: |
|
Sonstiges: |
|