Haematological response of patients with myelodysplastic syndrome to antithymocyte globulin is associated with a loss of lymphocyte-mediated inhibition of CFU-GM and alterations in T-cell receptor Vβ profiles
In: British journal of haematology, Jg. 102 (1998), Heft 5, S. 1314-1322
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academicJournal
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Zugriff:
We have demonstrated that 44% of myelodysplastic syndrome (MDS) patients with cytopenia have a haematological response to antithymocyte globulin (ATG). Three ATG responders and two non-responders with refractory anaemia were further studied for lymphocyte-mediated inhibition of bone marrow using a standard CFU-GM assay. In responders. peripheral blood lymphocytes (PBL) added at a 5:1 ratio suppressed CFU-GM by 54 ± 9% (P= 0.04) and was reversed by ATG treatment. Pre-treatment marrow depleted of CD 3 lymphocytes, increased CFU-GM by 32% (P=0.02) in an ATG responder, but not in a non-responder. CD3 lymphocytes from 6-month post-treatment marrow did not inhibit pre-treatment CFU-GM, indicating ATG had affected the T cells. Pre-treatment marrow depleted of CD8 lymphocytes, increased CFU-GM by 60% (P=0.01) and 49% (P=0.03) in two ATG responders, but not in a non-responder. Inhibition required cell-cell interaction through MHC I. TCR Vβ families, analysed by SSCP, changed from clonal to polyclonal in one ATG responder after 6 months, but clones persisted in a non-responder. These results indicate patients with refractory anaemia who respond to ATG have CD8 T-cell clones that mediate MHC-I-restricted suppression of CFU-GM which are replaced by polyclonal T cells that do not suppress CFU-GM after ATG treatment.
Titel: |
Haematological response of patients with myelodysplastic syndrome to antithymocyte globulin is associated with a loss of lymphocyte-mediated inhibition of CFU-GM and alterations in T-cell receptor Vβ profiles
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Autor/in / Beteiligte Person: | MOLLDREM, J. J ; YIN ZHENG, JIANG ; STETLER-STEVENSON, M ; MAVROUDIS, D ; HENSEL, N ; BARRETT, J |
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Zeitschrift: | British journal of haematology, Jg. 102 (1998), Heft 5, S. 1314-1322 |
Veröffentlichung: | Oxford: Blackwell, 1998 |
Medientyp: | academicJournal |
Umfang: | print, 20 ref |
ISSN: | 0007-1048 (print) |
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