Characterization of a Glutenin-Specific Serine Proteinase of Sunn Bug Eurygaster integricepts Put

KONAREV, Alexander V ; BEAUDOIN, Frédéric ; et al.

In: Journal of agricultural and food chemistry (Print), Jg. 59 (2011), Heft 6, S. 2462-2470

academicJournal - print, 46 ref

Glutenin hydrolyzing proteinases (GHPs) have been purified, by affinity chromatography, from wheat seeds damaged by the Sunn bug Eurygaster integriceps (Hemiptera, Scutelleridae). A 28 kDa protein was partially sequenced by mass spectrometry and Edman degradation which showed homology to serine proteases from various insects. Three full length clones were obtained from cDNA isolated from Sunn bug salivary glands using degenerate PCR based on the sequences obtained. The cleavage site of the protease was determined using recombinant and synthetic peptides and shown to be between the consensus hexapeptide and nonapeptide repeat motifs present in the high molecular weight subunits of wheat glutenin (PGQGQQ^GYYPTSLQQ). Homology models were generated for the three proteinases identified in this study using the high resolution X-ray structure of a crayfish (Pontastacus leptodactylus) trypsin complexed with a peptide inhibitor as template (PDB accession 2F91). The novel specificity of this protease may find applications in both fundamental and applied studies.

Titel:	Characterization of a Glutenin-Specific Serine Proteinase of Sunn Bug Eurygaster integricepts Put
Autor/in / Beteiligte Person:	KONAREV, Alexander V ; BEAUDOIN, Frédéric ; MARSH, Justin ; VILKOVA, Nina A ; NEFEDOVA, Ludmila I ; SIVRI, Dilek ; KÖKSEL, Hamit ; SHEWRY, Peter R ; LOVEGROVE, Alison
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Journal of agricultural and food chemistry (Print), Jg. 59 (2011), Heft 6, S. 2462-2470
Veröffentlichung:	Washington, DC: American Chemical Society, 2011
Medientyp:	academicJournal
Umfang:	print, 46 ref
ISSN:	0021-8561 (print)
Schlagwort:	Agronomy, agriculture, phytopathology Agronomie, agriculture, phytopathologie Food science technology Sciences technologies alimentaires Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Industries agroalimentaires Food industries Industries des céréales et produits de cuisson Cereal and baking product industries Protéines d'origine animale, végétale, fongique, microbienne, algues et levures aliments Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts Aminoacide Aminoacid Aminoácido Arthropoda Enzyme Enzima Heteroptera Hydrolases Insecta Invertebrata Pentatomidae Protéine végétale Plant protein Proteína vegetal Caractérisation Characterization Caracterización Eurygaster Gluten Gluténine Glutenin Glutenina Modélisation Modeling Modelización Peptidases Site clivage Cleavage site Sitio clivaje Site spécifique Site specificity Sitio específico Sérine Serine Serina Eurygaster intrigriceps Gluten hydrolyzing protease cleavage site specificity molecular modeling
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: All-Russian Institute for Plant Protection (VIZR), 3 Podbelsky, Pushkin, St, Petersburg 196608, Russian Federation ; Rothamsted Research, Harpenden, Hertfordshire AL5 2JQ, United Kingdom ; Food Engineering Department of Faculty of Engineering, Hacettepe University, 06532 Beytepe, Ankara, Turkey Rights: Copyright 2015 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Agrifood industries

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