On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis
In: Journal of chromatography. B, Jg. 879 (2011), Heft 11-12, S. 804-810
academicJournal
- print, 25 ref
Zugriff:
An integrated platform consisting of monolithic immobilized pH gradient-based capillary isoelectric focusing (M-IPG CIEF) and capillary zone electrophoresis (CZE) coupled by a partially etched porous interface was established. Since carrier ampholytes (CAs) were immobilized on monolith in M-IPG CIEF to form a stable pH gradient, subsequent depletion of CAs at the interface to prevent the interference on CZE separation and detection were avoided. Moreover, a partially etched porous capillary column, which was facile for fabrication and durable for operation, was exploited as the interface to combine M-IPG CIEF and CZE. The RSD values in terms of the migration time for M-IPG CIEF separation, transfer protein from the first dimension to the second dimension, and CZE separation, were 2.4%, 3.9% and 2.3%, respectively. With a 6-protein mixture as the sample, two-dimensional capillary electrophoresis (2D-CE) separation was successfully completed within 116 min, yielding a peak capacity of ~200 even with minute sample amount down to 5.0 μg/mL. The limit of detection was 0.2 μg/mL. In addition, proteins extracted from milk were used to test the performance of such a 2D-CE separation platform. We expect that such a novel 2D-CE system would provide a promising tool for protein separation with high throughput and high peak capacity.
Titel: |
On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis
|
---|---|
Autor/in / Beteiligte Person: | TINGTING, WANG ; JUNFENG, MA ; SHUAIBIN, WU ; LIANGLIANG, SUN ; HUIMING, YUAN ; LIHUA, ZHANG ; ZHEN, LIANG ; YUKUI, ZHANG |
Link: | |
Zeitschrift: | Journal of chromatography. B, Jg. 879 (2011), Heft 11-12, S. 804-810 |
Veröffentlichung: | Amsterdam: Elsevier, 2011 |
Medientyp: | academicJournal |
Umfang: | print, 25 ref |
ISSN: | 1570-0232 (print) |
Schlagwort: |
|
Sonstiges: |
|