Fetal RHD genotype detection from circulating cell-free fetal DNA in maternal plasma in non-sensitized RhD negative women
In: Prenatal diagnosis, Jg. 31 (2011), Heft 8, S. 802-808
Online
academicJournal
- print, 3/4 p
Zugriff:
Objective To examine the performance of the SensiGene Fetal RHD Genotyping Laboratory Developed Test (RHD Genotyping LDT) using circulating cell-free fetal DNA (ccff DNA) extracted from maternal plasma. Methods ccff DNA was extracted from maternal blood from non-sensitized women with singleton pregnancies in two cohorts, one with a serotype reference (11-13 weeks' gestation) and one with the reference source (6-30 weeks' gestation). The presence of three RHD exon sequences (exons 4, 5, 7), the psi-pseudogene, three Y-chromosome sequences (SRY, DBY and TTTY2), and the X/Y-chromosome TGIF gene control were determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry—the RHD Genotyping LDT. Results The cohort with a serotype RhD reference showed correct classification in 201 of 207 patients, a test accuracy of 97.1%, with a sensitivity and specificity for prediction of RhD serotype of 97.2 and 96.8%, respectively. The cohort with a genotype RHD reference showed correct classification in 198 of 199 patients, indicating a test accuracy of 99.5% with a sensitivity and specificity for prediction of RHD genotype of 100.0 and 98.3%, respectively. Conclusion Fetal RHD genotyping can accurately be determined using ccff DNA in the first and second trimesters of pregnancy.
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Fetal RHD genotype detection from circulating cell-free fetal DNA in maternal plasma in non-sensitized RhD negative women
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Autor/in / Beteiligte Person: | BOMBARD, Allan T ; AKOLEKAR, Ranjit ; FARKAS, Daniel H ; VANAGTMAEL, Anna L ; AQUINO, Frank ; OETH, Paul ; NICOLAIDES, Kypros H |
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Zeitschrift: | Prenatal diagnosis, Jg. 31 (2011), Heft 8, S. 802-808 |
Veröffentlichung: | Chichester: Wiley, 2011 |
Medientyp: | academicJournal |
Umfang: | print, 3/4 p |
ISSN: | 0197-3851 (print) |
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