Mass Spectrometry Based Metabolomics to Identify Potential Biomarkers for Resistance in Barley against Fusarium Head Blight (Fusarium graminearum)

KUMARASWAMY, Kenchappa G ; KUSHALAPPA, Ajjamada C ; et al.

In: Journal of chemical ecology, Jg. 37 (2011), Heft 8, S. 846-856

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Resistance in Triticeae to fusarium head blight (FHB) is quantitatively inherited. Metabolomics as a tool was used to better understand the mechanisms of resistance and to identify potential FHB resistance biomarker metabolites in barley. Five FHB-resistant two-row barley genotypes (CIho 4196, Zhedar-1, Zhedar-2, Fredrickson, and Harbin-2r) and one FHB-susceptible genotype (CH 9520― 30) were each inoculated with either pathogen-suspension or mock-solution. Disease severity, quantified as the proportion of spikelets diseased, varied among genotypes, being the greatest in CH 9520―30. Spikelets were sampled, metabolites extracted with aqueous methanol, and analyzed using an LC-ESI-LTQ-Orbitrap system. A pair wise, resistant vs. susceptible, t-test identified 1774 significant treatment peaks. Canonical discriminant analysis of peak abundance allowed the genotypes to be sorted into three clusters: (i) CH9520-30, (ii) Harbin-2r, (iii) the remaining four genotypes. The t-test was further used to identify resistance-related (RR) and pathogenesis-related (PR) metabolites. The pathogen-produced virulence factor deoxynivalenol (DON), and its detoxification product, DON-3-O-glucoside (D3G) were designated as resistance indicator (RI) metabolites. Metabolites (RR, PR, or RI) occurring in at least two resistant genotypes, showing a two-fold or greater abundance in resistant vs. susceptible lines, and also known to have plant defense functions were selected as potential FHB resistance biomarker metabolites. These included phenylalanine, p-coumaric acid, jasmonate, linolenic acid, total DON produced (TDP), and the proportion of DON converted to D3G (PDC). Total DON was the lowest in CIho 4196, while PDC was the highest in Zhedar-2. The application of RR, PR, and RI metabolites as potential biomarkers to enhance resistance is discussed.

Titel:	Mass Spectrometry Based Metabolomics to Identify Potential Biomarkers for Resistance in Barley against Fusarium Head Blight (Fusarium graminearum)
Autor/in / Beteiligte Person:	KUMARASWAMY, Kenchappa G ; KUSHALAPPA, Ajjamada C ; CHOO, Thin M ; DION, Yves ; RIOUX, Sylvie
Link:	Volltext (PDF) View record from PASCAL Archive
Zeitschrift:	Journal of chemical ecology, Jg. 37 (2011), Heft 8, S. 846-856
Veröffentlichung:	New York, NY: Springer, 2011
Medientyp:	academicJournal
Umfang:	print, 1 p.1/4
ISSN:	0098-0331 (print)
Schlagwort:	Ecology Ecologie Environment Environnement Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Ecologie animale, vegetale et microbienne Animal, plant and microbial ecology Ecologie animale et végétale Animal and plant ecology Généralités General aspects Ecologie appliquée Applied ecology Ecotoxicologie, effets biologiques de la pollution Ecotoxicology, biological effects of pollution Techniques Angiospermae Ascomycota Fungi Imperfecti Fungi Gramineae Monocotyledones Plante céréalière Cereal crop Planta cerealista Spermatophyta Fusarium graminearum Gibberella zeae Hordeum vulgare Marqueur biologique Biological marker Marcador biológico Orge Barley Cebada Sensibilité résistance Sensitivity resistance Sensibilidad resistencia Spectrométrie masse Mass spectrometry Espectrometría masa Tête Head Cabeza Barley · Biomarkers FHB Functional genomics Metabolomics · · DON
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Plant Science Department, McGill University, Sainte-Anne-de-Bellevue, QC H9X 3V9, Canada ; Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, 960 Carling Ave, Ottawa, ON K1A 0C6, Canada ; Centre de recherche sur les grains inc, 740 chemin Trudeau, Saint-Mathieu-de-Beloeil, QC J3G 4S5, Canada ; Centre de recherche sur les grains inc, 2700 rue Einstein, Ste. Foy, QC G1P 3W8, Canada Rights: Copyright 2015 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Animal, vegetal and microbial ecology

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