Suppressive effect of enzymatically modified isoquercitrin on phenobarbital-induced liver tumor promotion in rats
In: Archives of toxicology, Jg. 85 (2011), Heft 11, S. 1475-1484
Online
academicJournal
- print, 1 p.3/4
Zugriff:
To investigate the effect of enzymatically modified isoquercitrin (EMIQ) on hepatocellular tumor promotion induced by phenobarbital (PB), male rats were administered a single intraperitoneal injection of 200 mg/ kg N-diethylnitrosamine (DEN) and then fed with a diet containing PB (500 ppm) for 8 weeks, with or without EMIQ (2,000 ppm) in the drinking water. One week after PB administration, rats underwent a two-thirds partial hepatectomy. The PB-induced increase in the number and area of glutathione S-transferase placental form-positive foci and the proliferating cell nuclear antigen-positive ratio was significantly suppressed by EMIQ. Real-time reverse transcription―polymerase chain reaction analysis revealed increases in mRNA expression levels of Cyp2b2 and Mrp2 in the DEN-PB and DEN-PB-EMIQ groups compared with the DEN-alone group, while the level of Mrp2 decreased in the DEN-PB-EMIQ group compared with the DEN-PB group. There were no significant changes in microsomal reactive oxygen species (ROS) production and oxidative stress markers between the DEN-PB and DEN-PB-EMIQ groups. Immunohistochemically, the constitutive active/ androstane receptor (CAR) in the DEN-PB group was clearly localized in the nuclei, but its immunoreactive intensity was decreased in the DEN-PB-EMIQ group. These results indicate that EMIQ suppressed the liver tumor-promoting activity of PB by inhibiting nuclear translocation of CAR, and not by suppression of oxidative stress.
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Suppressive effect of enzymatically modified isoquercitrin on phenobarbital-induced liver tumor promotion in rats
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Autor/in / Beteiligte Person: | MORITA, Reiko ; SHIMAMOTO, Keisuke ; ISHII, Yuji ; KUWATA, Kazunori ; OGAWA, Bun-Ichiro ; IMAOKA, Masako ; HAYASHI, Shim-Mo ; SUZUKI, Kazuhiko ; SHIBUTANI, Makoto ; MITSUMORI, Kunitoshi |
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Zeitschrift: | Archives of toxicology, Jg. 85 (2011), Heft 11, S. 1475-1484 |
Veröffentlichung: | Heidelberg: Springer, 2011 |
Medientyp: | academicJournal |
Umfang: | print, 1 p.3/4 |
ISSN: | 0340-5761 (print) |
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