Rhesus monkey TRIM5α represses HIV-1 LTR promoter activity by negatively regulating TAK1/TAB1/TAB2/TAB3-complex-mediated NF-κB activation
In: Archives of virology, Jg. 156 (2011), Heft 11, S. 1997-2006
Online
academicJournal
- print, 45 ref
Zugriff:
TRIM5α has been identified as the main restriction factor responsible for resistance of Old World monkey cells to HIV-1 infection. The precise mechanism of viral inhibition by TRIM5α remains elusive but appears to occur in multiple ways. Here, we report that rhesus monkey TRIM5α (TRIM5αrh) can represses HIV-1 LTR promoter activity by negatively regulating TAK1/TAB1/ TAB2/TAB3-complex-mediated NF-κB activation when TRIM5αrh is overexpressed. We show that the overexpressed TRIM5αrh can interact with the TAK1/TAB1/ TAB2/TAB3 complex by binding to TAB1 and promotes the degradation of TAB2 within the complex via the lysosomal degradation pathway. Subsequently, TRIM5αrh lowers the IKKα protein level and inhibits NF-κB p65 phosphorylation, and knockdown of TRIM5αrh expression by small interfering RNA in TRIM5αrh-overexpressing cells can abolish this inhibition. Finally, the inhibition of p65 phosphorylation results in the repression of HIV-1 LTR promoter activity. Taken together, these findings indicate that TRIM5αrh plays a previously unrecognized role in repressing HIV-1 transcription by inhibiting TAK1/ TAB1/TAB2/TAB3-complex-mediated NF-κB activation when TRIM5αrh is overexpressed.
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Rhesus monkey TRIM5α represses HIV-1 LTR promoter activity by negatively regulating TAK1/TAB1/TAB2/TAB3-complex-mediated NF-κB activation
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Autor/in / Beteiligte Person: | JIAN, GONG ; SHEN, Xi-Hui ; HUI, QIU ; CHAO, CHEN ; YANG, Rong-Ge |
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Zeitschrift: | Archives of virology, Jg. 156 (2011), Heft 11, S. 1997-2006 |
Veröffentlichung: | Wien: Springer, 2011 |
Medientyp: | academicJournal |
Umfang: | print, 45 ref |
ISSN: | 0304-8608 (print) |
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