From SELDI-TOF MS to protein identification by on-chip elution
In: Journal of proteomics (Print), Jg. 74 (2011), Heft 12, S. 2995-2998
academicJournal
- print, 15 ref
Zugriff:
SELDI-TOF MS has been demonstrated as a powerful tool for biomarker discovery. However, a major disadvantage of SELDI-TOF MS is the lack of direct identification of the discriminatory peaks discovered. We describe a novel experimental identification strategy where peptides/proteins captured to a weak cation exchange ProteinArray surface (CM10) are eluted, and thereafter identified by utilizing a sensitive LC―MS/MS (i.e. LTQ Orbitrap). A mixture of four known proteins was used to test the novel experimental approach described, and all four proteins were successfully identified. Additionally, a biomarker candidate previously discovered in plasma of Atlantic cod (Gadus morhua) by SELDI-TOF MS was identified. Thus, this study indicated that a combination of on-chip elution and a highly sensitive LC―MS/MS system can be an alternative approach to identify biomarker candidates discovered by use of SELDI-TOF MS.
Titel: |
From SELDI-TOF MS to protein identification by on-chip elution
|
---|---|
Autor/in / Beteiligte Person: | MOELAND NILSEN, Mari ; ULEBERG, Kai-Erik ; JANSSEN, Emiel A. M ; BAAK, Jan P. A ; KETIL ANDERSEN, Odd ; HJELLE, Anne |
Link: | |
Zeitschrift: | Journal of proteomics (Print), Jg. 74 (2011), Heft 12, S. 2995-2998 |
Veröffentlichung: | Kidlington: Elsevier, 2011 |
Medientyp: | academicJournal |
Umfang: | print, 15 ref |
ISSN: | 1874-3919 (print) |
Schlagwort: |
|
Sonstiges: |
|