Effective Treatment of Refractory CMV Reactivation After Allogeneic Stem Cell Transplantation With In Vitro-generated CMV pp65-specific CD8+ T-cell Lines
In: Journal of immunotherapy (1997), Jg. 35 (2012), Heft 8, S. 621-628
academicJournal
- print, 29 ref
Zugriff:
To treat patients with refractory cytomegalovirus (CMV) reactivation after allogeneic stem cell transplantation, a phase I/II clinical study on adoptive transfer of in vitro-generated donor-derived or patient-derived CMV pp65-specific CD8+ T-cell lines was performed. Peripheral blood mononuclear cells from CMV seropositive donors or patients were stimulated with HLA-A*0201-restricted and/ or HLA-B*0702-restricted CMV pp65 peptides (NLV/TPR) and 1 day after stimulation interferon-γ)-producing cells were enriched using the CliniMACS Cytokine Capture System (interferon-γ), and cultured with autologous feeders and low-dose interluekin-2. After 7―14 days of culture, quality controls were performed and the CMV-specific T-cell lines were administered or cryopreserved. The T-cell lines generated contained 0.6-17 x 106 cells, comprising 54%―96% CMV pp65-specific CD8 + T cells, and showed CMV-specific lysis of target cells. Fifteen CMV-specific T-cell lines were generated of which 8 were administered to patients with refractory CMV reactivation. After administration, no acute adverse events and no graft versus host disease were observed and CMV load disappeared. In several patients, a direct relation between administration of the T-cell line and the in vivo appearance of CMV pp65-specific T cells could be documented. In conclusion, administration of CMV pp65-specific CD8+ T-cell lines was found to be feasible and safe, and enduring efficacy of administered CMV pp65-specific CD8+ T-cell lines could be demonstrated.
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Effective Treatment of Refractory CMV Reactivation After Allogeneic Stem Cell Transplantation With In Vitro-generated CMV pp65-specific CD8+ T-cell Lines
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Autor/in / Beteiligte Person: | MEIJ, Pauline ; JEDEMA, Inge ; MARIJT, Erik W. A ; VON DEM BORNE, Peter A ; LANKESTER, Arjan C ; HALKES, Constantijn J. M ; FALKENBURG, J. H. Frederik ; ZANDVLIET, Maarten L ; VAN DER HEIDEN, Pim L. J ; VAN DE MEENT, Marian ; VAN EGMOND, H. M. Esther ; VAN LIEMPT, Ellis ; HOOGSTRATEN, Conny ; KRUITHOF, Simone ; VELD, Sabrina |
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Zeitschrift: | Journal of immunotherapy (1997), Jg. 35 (2012), Heft 8, S. 621-628 |
Veröffentlichung: | Hagerstown, MD: Lippincott Williams & Wilkins, 2012 |
Medientyp: | academicJournal |
Umfang: | print, 29 ref |
ISSN: | 1524-9557 (print) |
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