Capillary isoelectric focusing with anionic coated capillaries
In: Journal of chromatography, Jg. 767 (1997), Heft 1-2, S. 231-239
academicJournal
- print, 15 ref
Zugriff:
Capillary isoelectric focusing (cIEF) is a powerful analytical technique for the separation of proteins. cIEF can be performed in capillaries with reduced electroosmotic flow, or in capillaries with controlled electroosmotic flow. This work introduces an alternative method for cIEF with controlled electroosmotic flow through the use of an anionic coated capillary. The method is shown to be applicable to basic, neutral and acidic proteins, with some peak broadening for acidic proteins. It also proves to have good reproducibility with R.S.D.s around 3%, a linear relationship between migration time and isoelectric point and quantitative capability. The total analysis time is shorter and sample preparation is simplified over other cIEF methods.
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Capillary isoelectric focusing with anionic coated capillaries
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Autor/in / Beteiligte Person: | WHYNOT, D. M ; HARTWICK, R. A ; BANE, S |
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Zeitschrift: | Journal of chromatography, Jg. 767 (1997), Heft 1-2, S. 231-239 |
Veröffentlichung: | Amsterdam: Elsevier, 1997 |
Medientyp: | academicJournal |
Umfang: | print, 15 ref |
ISSN: | 0021-9673 (print) |
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