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In human milk zinc (Zn) is bound to proteins and low molecular mass (LMM) ligands. Numerous investigations demonstrated that Zn bioavailability in human milk is for infant much higher than in cow's milk. It was presumed that in the LMM human milk fraction highly bioavailable Zn-citrate prevails. However, literature data are controversial regarding the amount of Zn-citrate in human milk since analytical procedures reported were not quantitative. So, complex investigation was carried out to develop analytical method for quantitative determination of this biologically important molecule. Studies were performed within the pH range 5-7 by the use of synthetic solutions of Zn-citrate prepared in HEPES, MOPS and MES buffers. Zn-citrate was separated on weak anion-exchange convective interaction media (CIM) diethylaminoethyl (DEAE) monolithic chromatographic column using NH4NO3 as an eluent. Separated Zn species were determined by flame atomic absorption spectrometry (FAAS) or inductively coupled plasma mass spectrometry (ICP-MS). Quantitative separation of Zn-citrate complexes ([Zn(Cit)]― and [Zn(Cit)2]4―; column recoveries 94-102%) and good repeatability and reproducibility of results with relative standard deviation (RSD ± 3.0%) were obtained. In fractions under the chromatographic peaks Zn-binding ligand was identified by electrospray ionization tandem mass spectrometry (ESI-MS-MS). Limits of detection (LOD) for determination of Zn-citrate species by CIM DEAE-FAAS and CIM DEAE-ICP-MS were 0.01 μg Zn mL―1 and 0.0005 μg Zn mL―1, respectively. Both techniques were sensitive enough for quantification of Zn-citrate in human milk. Results demonstrated that about 23% of total Zn was present in the LMM milk fraction and that LMM-Zn corresponded to Zn-citrate. The developed speciation method represents a reliable analytical tool for investigation of the percentage and the amount of Zn-citrate in human milk.

Titel:	Determination of Zn-citrate in human milk by CIM monolithic chromatography with atomic and mass spectrometry detection
Autor/in / Beteiligte Person:	MILACIC, Radmila ; AJLEC, Dejan ; ZULIANI, Tea ; ZIGON, Dušan ; SCANCAR, Janez
Link:	E-Journal im Bestand der UB Hagen? View record from PASCAL Archive
Zeitschrift:	Talanta (Oxford), Jg. 101 (2012), S. 203-210
Veröffentlichung:	Amsterdam: Elsevier, 2012
Medientyp:	academicJournal
Umfang:	print, 37 ref
ISSN:	0039-9140 (print)
Schlagwort:	Analytical chemistry Chimie analytique Sciences exactes et technologie Exact sciences and technology Chimie Chemistry Méthodes chromatographiques et méthodes physiques associées à la chromatographie Chromatographic methods and physical methods associated with chromatography Autres méthodes chromatographiques Other chromatographic methods Méthodes spectrométriques et optiques Spectrometric and optical methods Artiodactyla Mammalia Ungulata Vertebrata Analyse chimique Chemical analysis Análisis químico Analyse quantitative Quantitative analysis Análisis cuantitativo Biodisponibilité Bioavailability Biodisponibilidad Bovin Bovine Bovino Chromatographie colonne Column chromatography Cromatografía columna Citrate Citrato Détection Detection Detección Ecart type Standard deviation Desviación típica Echange anion Anion exchange Cambio aniónico Electrospray Homme Human Hombre Ionisation chimique Chemical ionization Ionización química Lait Milk Leche Ligand Ligando Masse moléculaire Molecular mass Masa molecular Microsonde laser Laser microprobe Microsonda láser Molécule Molecules Molécula Méthode analytique Analytical method Método analítico Méthode couplée Coupled method Método acoplado Pic Peak Pico Protéine Protein Proteína Reproductibilité Reproducibility Reproductividad Répétabilité Repeatability Repetibilidad Spectrométrie ICP Inductive coupling plasma spectrometry Espectrometría ICP Spectrométrie absorption atomique Atomic absorption spectrometry Espectrometría absorción atómica Spectrométrie flamme Flame spectrometry Espectrometría llama Spectrométrie masse tandem Mass spectrometry MS/MS Espectrometría masa en tándem Spectrométrie masse Mass spectrometry Espectrometría masa Utilisation Use Uso Zinc pH Chemical speciation Human milk Monolithic chromatography Spectrometry techniques Zn-citrate
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Department of Environmental Sciences, Jožef Stefan Institute, Jamova 39, 1000 Ljubljana, Slovenia Rights: Copyright 2014 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Analytical chemistry

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Determination of Zn-citrate in human milk by CIM monolithic chromatography with atomic and mass spectrometry detection