A case of ganciclovir-resistant cytomegalovirus (CMV) retinitis in a patient with AIDS : longitudinal molecular analysis of the CMV viral load and viral mutations in blood compartments
In: AIDS (London), Jg. 11 (1997), Heft 7, S. 867-873
Online
academicJournal
- print, 24 ref
Zugriff:
Objectives: To study the temporal relationships between cytomegalovirus (CMV) viral load and specific UL97 mutations in polymorphonuclear leukocytes (PMNL) and plasma samples from a patient with AIDS who developed ganciclovir-resistant CMV retinitis. Methods: Sequential PMNL and plasma samples were analysed for determination of the CMV viral load using non-molecular methods and a quantitative polymerase chain reaction (PCR) assay. Screening of the same samples for the most common mutations conferring ganciclovir resistance was performed using nested PCR and restriction enzyme analysis. Results: At the time of progression of CMV retinitis (after 6 months of ganciclovir), a rapid increase in the CMV DNA load was found in both PMNL and plasma samples. This increase paralleled the emergence of a specific mutation (V794) in the same samples and recovery of ganciclovir-resistant blood isolates. In this patient, however, the only tests that substantially predicted the progression of CMV disease were the quantitative PCR assay using PMNL and to a lesser extent the pp65 antigenemia assay. Conclusions: Quantitative evaluation of the CMV viral load in PMNL using sensitive assays such as PCR appears to be a promising approach for monitoring antiviral therapy in subjects with AIDS. In addition, common mutations conferring ganciclovir resistance can be detected directly in PMNL and plasma samples.
Titel: |
A case of ganciclovir-resistant cytomegalovirus (CMV) retinitis in a patient with AIDS : longitudinal molecular analysis of the CMV viral load and viral mutations in blood compartments
|
---|---|
Autor/in / Beteiligte Person: | BOIVIN, G ; GILBERT, C ; MORISSETTE, M ; HANDFIELD, J ; GOYETTE, N ; BERGERON, M. G |
Link: | |
Zeitschrift: | AIDS (London), Jg. 11 (1997), Heft 7, S. 867-873 |
Veröffentlichung: | Hagerstown, MD: Lippincott Williams & Wilkins, 1997 |
Medientyp: | academicJournal |
Umfang: | print, 24 ref |
ISSN: | 0269-9370 (print) |
Schlagwort: |
|
Sonstiges: |
|