Biodistribution of 111In-labelled SCN-bz-DTPA-BC-2 MAb following loco-regional injection into glioblastomas
In: International journal of cancer, Jg. 71 (1997), Heft 5, S. 810-816
Online
academicJournal
- print, 23 ref
Zugriff:
We analyzed the biodistribution of the 111In-labelled murine anti-tenascin-C MAb BC-2 after intralesional injection in IS glioblastoma patients. The activated ligand DTPA was conjugated via the isothiocyanato-benzyl group onto BC-2. Conjugates were labelled with 111In, displaying immunoreactivity greater than 90% and labelling efficiency of 99 ± 1%. In contrast to i.v. injections, excellent tumor uptake was obtained by direct intralesional injection of conjugates that showed only slow systemic release. In serum, conjugates were found to be intact; in urine, only low-molecular-weight decay products were detected. In 8 patients, outflow from the site of injection into systemic circulation was low; daily activity in the serum and urine was found to be below 2% of the total injected radioactivity; most of the injected activity was retained within the tumor, resulting in effective half-lives of 58 ± 5 hr. In contrast, higher outflow up to 10% of regionally injected 111In-DTPA-BC-2 MAb into systemic circulation resulted in considerable shortening of the effective half-lives to 20 to 40 hr in 7 patients. This outflow was found to correlate with tumor size and blood/brain barrier disruption. In one patient, H PLC analysis of tumor cyst fluid 3 and 6 days after intralesional injection revealed conjugates to be intact and allowed the estimate of about 70% of the total injected 111In-DTPA-BC-2 to be confined to tumor tissue. We conclude that different outflow patterns can be observed following locoregional injection of 111In-DTPA-BC-2, leading to considerable variations in the effective half-lives of isotopes within the tumor, requiring adjustment of the radiation dose in therapeutic trials.
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Biodistribution of 111In-labelled SCN-bz-DTPA-BC-2 MAb following loco-regional injection into glioblastomas
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Autor/in / Beteiligte Person: | MERLO, A ; JERMANN, E ; HAUSMANN, O ; CHIQUET-EHRISMANN, R ; PROBST, A ; LANDOLT, H ; MAECKE, H. R ; MUELLER-BRAND, J ; GRATZL, O |
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Zeitschrift: | International journal of cancer, Jg. 71 (1997), Heft 5, S. 810-816 |
Veröffentlichung: | New York, NY: Wiley-Liss, 1997 |
Medientyp: | academicJournal |
Umfang: | print, 23 ref |
ISSN: | 0020-7136 (print) |
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