De Novo Correction of Mass Measurement Error in Low Resolution Tandem MS Spectra for Shotgun Proteomics
In: Journal of the American Society for Mass Spectrometry, Jg. 23 (2012), Heft 12, S. 2075-2082
academicJournal
- print, 35 ref
Zugriff:
We report an algorithm designed for the calibration of low resolution peptide mass spectra. Our algorithm is implemented in a program called FineTune, which corrects systematic mass measurement error in 1 min, with no input required besides the mass spectra themselves. The mass measurement accuracy for a set of spectra collected on an LTQ-Velos improved 20-fold from ―0.1776±0.0010m/z to 0.0078±0.0006m/z after calibration (avg±95 % confidence interval). The precision in mass measurement was improved due to the correction of non-linear variation in mass measurement accuracy across the m/z range.
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De Novo Correction of Mass Measurement Error in Low Resolution Tandem MS Spectra for Shotgun Proteomics
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Autor/in / Beteiligte Person: | EGERTSON, Jarrett D ; ENG, Jimmy K ; BEREMAN, Michael S ; HSIEH, Edward J ; MERRIHEW, Gennifer E ; MACCOSS, Michael J |
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Zeitschrift: | Journal of the American Society for Mass Spectrometry, Jg. 23 (2012), Heft 12, S. 2075-2082 |
Veröffentlichung: | Amsterdam: Elsevier, 2012 |
Medientyp: | academicJournal |
Umfang: | print, 35 ref |
ISSN: | 1044-0305 (print) |
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