Assays for the analysis of P-glycoprotein in acute myeloid leukemia and CD34 subsets of AML blasts : Phenotypic and genotypic analyses of multidrug resistance (MDR) in clinical hospital practice
In: Leukemia, Jg. 11 (1997), Heft 7, S. 1160-1165
Online
academicJournal
- print, 47 ref
Zugriff:
Expression of the multidrug resistance (MDR) phenotype is an independent prognostic variable in acute myeloid leukemia. Approximately 43-57% of the patients have P-glycoprotein (P-gp) expression. A major drawback with the Interpretation of P-gp data in AML is the lack of coherence with different analytical assays. We have focused our efforts of P-gp detection on flow cytometry using a dual technique of P-gp staining with antibodies for the extracellular epitope (MRK16) and a functional analysis of P-gp using the rhodamine efflux assay and the effect of P-gp inhibitors such as SDZ PSC 833. This technique was combined with the staining of lineage-specific antigens such as CD34, CD56 and c-kit. In this way, various subsets of AML cells can be identified such as MRK 16+/-, CD34+/- blasts. These cells can be sorted for further analysis, such as the molecular expression of P-gp and other pleiotropic drug resistance genes.
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Assays for the analysis of P-glycoprotein in acute myeloid leukemia and CD34 subsets of AML blasts : Phenotypic and genotypic analyses of multidrug resistance (MDR) in clinical hospital practice
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Autor/in / Beteiligte Person: | SONNEVELD, P ; WIEMER, E |
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Zeitschrift: | Leukemia, Jg. 11 (1997), Heft 7, S. 1160-1165 |
Veröffentlichung: | London: Nature Publishing, 1997 |
Medientyp: | academicJournal |
Umfang: | print, 47 ref |
ISSN: | 0887-6924 (print) |
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