Evaluation of Cytomegalovirus (CMV)-Specific T-Cell Immunity for the Assessment of the Risk of Active CMV Infection in Non-Immunosuppressed Surgical and Trauma Intensive Care Unit Patients
In: Journal of medical virology, Jg. 85 (2013), Heft 10, S. 1802-1810
Online
academicJournal
- print, 1/4 p
Zugriff:
The current study was designed to assess the predictive value of the evaluation of cytomegalovirus (CMV)-specific T-cell immunity early following admission to the intensive care unit for inferring the risk of active CMV infection in non-immunosuppressed surgical and trauma patients. A total of 31 CMV-seropositive patients were included. Patients were screened for the presence of CMV DNA in plasma and in tracheal aspirates by real-time PCR. Enumeration of CMV pp65 and IE-1-specific IFN-γ CD8+ and CD4+ T cells was performed by flow cytometry for intracellular cytokine staining. Virological and immunological monitoring was conducted once or twice a week. Active CMV infection occurred in 17 out of 31 patients. Undetectable levels of pp65 and IE-1-specific IFN-γ CD8+ and CD4+ T-cell subsets cells were observed in 10 patients who developed active CMV infection and in one who did not (at a median of 2 days following ICU admission). Peak CMV DNA loads in both tracheal aspirates and plasma were substantially higher (P = 0.018 and P = 0.091, respectively) in patients with undetectable IFN-γ T-cell responses than in patients with detectable responses. The expansion of both CMV-specific T-cell subsets following detection of active CMV infection was demonstrated in 9 out of 14 patients with active CMV infection. In conclusion, the evaluation of CMV pp65 and IE-1-specific IFN-γ-producing CD8+ and CD4+ T cells early following ICU admission may allow the identification of patients most at risk of either having or developing an episode of active CMV infection, particularly those associated with high-level virus replication.
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Evaluation of Cytomegalovirus (CMV)-Specific T-Cell Immunity for the Assessment of the Risk of Active CMV Infection in Non-Immunosuppressed Surgical and Trauma Intensive Care Unit Patients
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Autor/in / Beteiligte Person: | CLARI, María A ; AGUILAR, Gerardo ; BENET, Isabel ; BELDA, Javier ; GIMENEZ, Estela ; BRAVO, Dayana ; CARBONELL, José A ; HENAO, Liliana ; NAVARRO, David |
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Zeitschrift: | Journal of medical virology, Jg. 85 (2013), Heft 10, S. 1802-1810 |
Veröffentlichung: | Hoboken, NJ: Wiley, 2013 |
Medientyp: | academicJournal |
Umfang: | print, 1/4 p |
ISSN: | 0146-6615 (print) |
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