Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells

KANDASAMY, Karthikeyan ; NARAYANAN, Karthikeyan ; et al.

In: Biomacromolecules, Jg. 15 (2014), Heft 6, S. 2067-2078

academicJournal - print, 33 ref

Clinical and industrial applications of human pluripotent stem cells (hPSC) require large amounts of cells that have been expanded under defined conditions. Labor-intensive techniques and ill-defined or expensive compounds and substrates are not applicable. Here we describe a chemically defined synthetic substrate consisting of polysulfone (PSF) membranes coated with polymerized 3,4-dihydroxy-L-phenylalanine (DOPA). DOPA/PSF is inexpensive and can be easily produced at various shapes and sizes. DOPA/PSF supports long-term self-renewal of undifferentiated human embryonic (hESC) and human induced pluripotent stem cells (hiPSC) under defined conditions. Pluripotency is maintained for at least 10 passages. Adhesion of hPSC to DOPA/PSF is mainly mediated by a specific integrin heterodimer. Proliferation and gene expression patterns on DOPA/PSF and control substrates are comparable. Labor-intensive cultivation methods and use of serum or coating with proteins are not required. Together, these features make DOPA/PSF attractive for applications where large-scale expansion of human pluripotent stem cells under defined conditions is essential.

Titel:	Polysulfone Membranes Coated with Polymerized 3,4-Dihydroxy-L-phenylalanine are a Versatile and Cost-Effective Synthetic Substrate for Defined Long-Term Cultures of Human Pluripotent Stem Cells
Autor/in / Beteiligte Person:	KANDASAMY, Karthikeyan ; NARAYANAN, Karthikeyan ; MING, NI ; CHAN, DU ; WAN, Andrew C. A ; ZINK, Daniele
Link:	View record from PASCAL Archive
Zeitschrift:	Biomacromolecules, Jg. 15 (2014), Heft 6, S. 2067-2078
Veröffentlichung:	Washington, DC: American Chemical Society, 2014
Medientyp:	academicJournal
Umfang:	print, 33 ref
ISSN:	1525-7797 (print)
Schlagwort:	Biochemistry, molecular biology, biophysics Biochimie, biologie moléculaire, biophysique Polymers, paint and wood industries Polymères, industries des peintures et bois Sciences exactes et technologie Exact sciences and technology Sciences appliquees Applied sciences Industrie des polymeres, peintures, bois Polymer industry, paints, wood Technologie des polymères Technology of polymers Formes d'application et semiproduits Forms of application and semi-finished materials Résines échangeuses et membranes Exchange resins and membranes Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Biotechnologie Biotechnology Méthodes. Procédés. Technologies Methods. Procedures. Technologies Cultures de cellules eucaryotes Eukaryotic cell cultures Cellules animales Animal cells Etablissement de nouvelles lignées, amélioration des méthodes de culture, cultures en masse Establishment of new cell lines, improvement of cultural methods, mass cultures Adhérence cellulaire Cell adhesion Adherencia celular Cellule embryonnaire Embryonic cell Célula embrionaria Cellule pluripotente Pluripotent cell Célula pluripotente Cellule souche Stem cell Célula primitiva Culture cellulaire Cell culture Cultivo celular Cyclopolymère Cyclopolymer Ciclopolímero Etude expérimentale Experimental study Estudio experimental Indole dérivé polymère Indole derivative polymer Indol derivado polímero Matériau revêtu Coated material Material revestido Membrane Membrana Multiplication cellulaire Cell proliferation Multiplicación celular Mécanisme Mechanism Mecanismo Polymère aromatique Aromatic polymer Polímero aromático Polymère fonctionnel Functional polymer Polímero funcional Sulfone polymère Sulfone polymer Sulfona polímero Support Soporte Autorenouvellement Dopamine polymère
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, The Nanos, Singapore 138669, Singapore Rights: Copyright 2015 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Biotechnology ; Polymer industry, paints, wood

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