New Citrate-Bicarbonate-Ethylenediaminetetraacetate (CBE) Method for Chemical Extraction of Hydrous Iron Oxides from Plant Root Surfaces

AZIZUR RAHMAN, M ; MAMUNUR RAHMAN, M ; et al.

In: Communications in soil science and plant analysis, Jg. 45 (2014), Heft 12-14, S. 1760-1771

academicJournal - print, 1 p.1/2

In the present study, the sodium citrate, sodium bicarbonate, and ethylenediaminetetraacetate (CBE) method was evaluated for iron (Fe) extraction from plant root surfaces and compared with the dithionite-citrate-bicarbonate (DCB) method. Iron plaque on root surfaces was induced by growing rice seedlings in soil with 1.8 mM Fe2+. Iron plaque was extracted following CBE and DCB methods. The effects of pH, temperature, and incubation time of these methods on Fe extraction from root surfaces were also examined. Iron extraction of CBE and DCB methods did not differ significantly (P < 0.05) at pH between 6 and 8, whereas Fe extraction decreased substantially for further increase of the pH of CBE and DCB solution. In some instances, there were significant differences between CBE and DCB methods in extracellular Fe extraction for temperature and incubation time. The average Fe extraction of CBE and DCB methods were 94% and 81%, respectively, indicating that CBE method would be a better choice for Fe extraction from plant roots. The recommended optimal conditions for CBE method are pH 8, volume of the solution 30 mL, incubation time 30 min, and solution temperature 22 ± 2 °C.

Titel:	New Citrate-Bicarbonate-Ethylenediaminetetraacetate (CBE) Method for Chemical Extraction of Hydrous Iron Oxides from Plant Root Surfaces
Autor/in / Beteiligte Person:	AZIZUR RAHMAN, M ; MAMUNUR RAHMAN, M ; HASEGAWA, H
Link:	View record from PASCAL Archive
Zeitschrift:	Communications in soil science and plant analysis, Jg. 45 (2014), Heft 12-14, S. 1760-1771
Veröffentlichung:	Philadelphia, PA: Taylor & Francis, 2014
Medientyp:	academicJournal
Umfang:	print, 1 p.1/2
ISSN:	0010-3624 (print)
Schlagwort:	Agronomy, agriculture, phytopathology Agronomie, agriculture, phytopathologie Plant biology and physiology Biologie et physiologie végétales Soil science, pedology Science du sol, pédologie Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Agronomie. Sciences du sol et productions vegetales Agronomy. Soil science and plant productions Chimie Chemistry Química Citrate Citrato Hydrogénocarbonate Hydrogencarbonates Hidrógenocarbonato Oxyde de fer Iron oxide Hierro óxido Racine Root Raíz CBE DCB Fe extraction Fe plaque plant roots
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Centre for Environmental Sustainability, School of the Environment, Faculty of Science, University of Technology Sydney, Broadway, Australia ; Graduate School of Natural Science and Technology, Kanazawa University, Kakuma, Kanazawa, Japan ; Bangladesh Rice Research Institute (BRRI), Gazipur, Bangladesh ; Institute of Science and Engineering, Kanazawa University, Kakuma, Kanazawa, Japan Rights: Copyright 2015 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Agronomy. Soil sciences and vegetal productions

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