In situ radiation sensitivity of recombinant human granulocyte colony-stimulating factor : Recruited murine circulating blood and bone marrow progenitors (colony-forming unit [CFU]-granulocyte-macrophage and CFU-megakaryocyte) : Evidence for possible biologic differences between mobilized blood and bone marrow

SCHEDING, S ; MEDIA, J. E ; et al.

In: Blood, Jg. 88 (1996), Heft 2, S. 472-478

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Increasing evidence especially stemming from peripheral blood progenitor transplantation studies points to a possible biologic difference between mobilized blood and bone marrow progenitor cells. The objective of this study was to compare the in situ radiation sensitivity of recombinant human granulocyte colony-stimulating factor (rhG-CSF)-recruited circulating granulopoietic (blood colony-forming unit-granulocyte-macrophage [CFU-GMblood]) and megakaryocytopoietic (blood CFU-megakaryocyte [CFU-Megblood]) progenitors, with the nonmobilized fraction remaining in the bone marrow (CFU-GMfemur and CFU-Megfemur). Splenectomized ? B6D2F1 mice received 50 μg/kg/d rhG-CSF daily for 8 days to induce high levels of circulating progenitors, followed by either total body X-irradiation (TBI) or X-irradiation of the chest (CI) with 62.5, 125, 250, or 500 cGy. Progenitor cells were assayed 24 hours after irradiation. Circulating CFUGM and CFU-Meg in the blood were decreased in a dose-dependent fashion by both TBI and Cl, with TBI causing greater damage than Cl. Average D0 values for TBI were 53 cGy for CFU-GMblood and 40 cGy for CFU-Megblood. D0 values for Cl were 90 cGy for CFU-GMblood and 140 cGy for CFU-Meg blood. As seen for blood progenitor cells, TBI caused a dose-dependent decrease of both CFU-GMfemur (D0, 136 cGy) and CFU-Megfemur (D0, 148 cGy). However, radiation-induced bone marrow progenitor cell kill was significantly lower when compared with blood progenitors. Despite the fact that circulating blood elements only received a fraction of the total dose administered as Cl, the extent of blood progenitor kill caused by Cl was higher than the effects of identical TBI doses on bone marrow CFU. The results of this study showed that rhG-CSF-recruited CFU-Megblood and CFU-GM blood were considerably more radiosensitive than femoral progenitors, thereby providing novel evidence for a biologic difference between rhG-CSF-recruited peripheral blood progenitors and the nonrecruited bone marrow CFU.

Titel:	In situ radiation sensitivity of recombinant human granulocyte colony-stimulating factor : Recruited murine circulating blood and bone marrow progenitors (colony-forming unit [CFU]-granulocyte-macrophage and CFU-megakaryocyte) : Evidence for possible biologic differences between mobilized blood and bone marrow
Autor/in / Beteiligte Person:	SCHEDING, S ; MEDIA, J. E ; KUKURUGA, M. A ; NAKEFF, A
Link:	Full Text Finder (Volltext) View record from PASCAL Archive
Zeitschrift:	Blood, Jg. 88 (1996), Heft 2, S. 472-478
Veröffentlichung:	Washington, DC: The Americain Society of Hematology, 1996
Medientyp:	academicJournal
Umfang:	print, 36 ref
ISSN:	0006-4971 (print)
Schlagwort:	Medical oncology Cancérologie Hematology Hématologie Sciences biologiques et medicales Biological and medical sciences Sciences biologiques fondamentales et appliquees. Psychologie Fundamental and applied biological sciences. Psychology Biophysique des tissus, organes et organismes Tissues, organs and organisms biophysics Effets biologiques des rayonnements Biological effects of radiation Rayonnements ionisants Ionizing radiations Cytokine Citoquina Mammalia Polypeptide Polipéptido Rodentia Vertebrata Animal Biophysique Biophysics Biofísica Cellule CFU-GM CFU-GM-Cell Célula CFU-GM Cellule CFU-Meg CFU-Meg-Cell Célula CFU-Meg Cellule hématopoïétique Hematopoietic cell Célula hematopoyética Cellule souche Stem cell Célula primitiva Etude comparative Comparative study Estudio comparativo Facteur stimulant colonie granulocyte Granulocyte colony stimulating factor Factor estimulante colonia granulocito Irradiation ionisante Ionizing irradiation Irradiación ionizante Mobilisation Mobilization Mobilización Moelle osseuse Bone marrow Médula ósea Radiobiologie Radiobiology Radiobiología Radiosensibilité Radiosensitivity Radiosensibilidad Sang Blood Sangre Souris Mouse Ratón
Sonstiges:	Nachgewiesen in: PASCAL Archive Sprachen: English Original Material: INIST-CNRS Document Type: Article File Description: text Language: English Author Affiliations: Division of Hematology and Oncology, Wayne State University, School of Medicine, Detroit, MI, United States Rights: Copyright 1996 INIST-CNRS ; CC BY 4.0 ; Sauf mention contraire ci-dessus, le contenu de cette notice bibliographique peut être utilisé dans le cadre d’une licence CC BY 4.0 Inist-CNRS / Unless otherwise stated above, the content of this bibliographic record may be used under a CC BY 4.0 licence by Inist-CNRS / A menos que se haya señalado antes, el contenido de este registro bibliográfico puede ser utilizado al amparo de una licencia CC BY 4.0 Inist-CNRS Notes: Biophysics of tissues, organs and organisms

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