Possible involvement of the lysine ε-aminotransferase gene (lat) in the expression of the genes encoding ACV synthetase (pcbAB) and isopenicillin N synthase (pcbC) in Streptomyces clavuligerus
In: Microbiology (Reading), Jg. 140 (1994), S. 3367-3377
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Zugriff:
Streptomyces clavuligerus produces the β-lactam antibiotics penicillin N, O-carbamoyldeacetylcephalosporin C and cephamycin C. We characterized a wildtype DNA region which restores antibiotic formation to a mutant strain named NP1, previously shown to exhibit depressed activities for two early enzymes of cephalosporin synthesis, δ-(L-α-aminoadipyl)-L-cysteinyl-D-valine synthetase (ACVS) and isopenicillin N synthase (IPNS). L-Lysine ε-aminotransferase (LAT) assays and α-AAA feeding experiments suggested that strain NP1 is a lat mutant. NP1 recovered LAT, ACVS and IPNS activities when transformed with the cloned region.
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Possible involvement of the lysine ε-aminotransferase gene (lat) in the expression of the genes encoding ACV synthetase (pcbAB) and isopenicillin N synthase (pcbC) in Streptomyces clavuligerus
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Autor/in / Beteiligte Person: | YU, H ; SERPE, E ; PIRET, J ; ROMERO, J ; COQUE, J.-J ; MAEDA, K ; OELGESCHLÄGER, M ; HINTERMANN, G ; LIRAS, P ; MARTIN, J.-F ; DEMAIN, A. L |
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Zeitschrift: | Microbiology (Reading), Jg. 140 (1994), S. 3367-3377 |
Veröffentlichung: | Reading: Society for General Microbiology, 1994 |
Medientyp: | academicJournal |
Umfang: | print, 1 p.1/4 12 |
ISSN: | 1350-0872 (print) |
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