Decrease in cytochrome P4502E1 as assessed by the rate of chlorzoxazone hydroxylation in alcoholics during the withdrawal phase
In: Alcoholism, clinical and experimental research, Jg. 19 (1995), Heft 2, S. 362-366
academicJournal
- print, 26 ref
Zugriff:
To evaluate cytochrome P4502E1 (CYP2E1) induction in alcoholics, the ratio of the concentrations of 6-hydroxychlorzoxazone (6-OH-CHZ) and chlorzoxazone (CHZ) was measured in blood 2 hr after CHZ ingestion using a HPLC method. This ratio was determined in controls and in alcoholic patients after 1, 2, 3, 4, 5, 8, and 21 days withdrawal. It was found to be 0.34±0.03 in 30 controls and 1.05±0.14 in 41 alcoholic patients within 2 days following ethanol withdrawal. This ratio decreased rapidly during withdrawal as attested by the short half-life of CYP2E1, which was found to be 2.5 days. Patient tesred for CHZ metabolism after 8 or 21 days alcohol abstinence displayed the same ratio as controls [0.35±0.03 (n=28) and 0.31±0.03 (n=34), respectively]. No comelation was observed between γ-glutamyltransferase, carbohydrate-deficient tranferrin values, the amount of alcohol consumed/day, and the 6-OH-CHZ/CHZ ratio. There was no influence of smoking on the rate of CHZ hydroxylation, because smokers displayed the same ratio as nonsmokers [0.33±0.025 (n=62) and 0.33±0.02 (n=30), respectively]. The CHZ hydroxylation ratio seems to be a good reflection of the hepatic and extrahepatic CYP2E1 activity in humans.
Titel: |
Decrease in cytochrome P4502E1 as assessed by the rate of chlorzoxazone hydroxylation in alcoholics during the withdrawal phase
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Autor/in / Beteiligte Person: | LUCAS, D ; MENEZ, C ; GIRRE, C ; BODENEZ, P ; HISPARD, E ; MENEZ, J.-F |
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Zeitschrift: | Alcoholism, clinical and experimental research, Jg. 19 (1995), Heft 2, S. 362-366 |
Veröffentlichung: | Baltimore, MD: Lippincott Williams & Wilkins, 1995 |
Medientyp: | academicJournal |
Umfang: | print, 26 ref |
ISSN: | 0145-6008 (print) |
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