C4 isoform of NADP-malate dehydrogenase : cDNA cloning and expression in leaves of C4, C3, and C3-C4 intermediate species of Flaveria
In: Plant physiology (Bethesda), Jg. 108 (1995), Heft 3, S. 1119-1126
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Zugriff:
In C4 plants of the NADP-malic enzyme type, an abundant, mesophyll cell-localized NADP-malate dehydrogenase (MDH) acts to convert oxaloacetate, the initial product of carbon fixation, to malate before it is shuttled to the bundle sheath. Since NADP-MDH has different but important roles in leaves of C3 and C4 plants, we have cloned and characterized a nearly full-length cDNA encoding NADP-MDH from Flaveria trinervia (C4) to permit comparative structure/expression studies within the genus Flaveria. The dicot genus Flaveria includes C3-C4 intermediate species, as well as C3 and C4 species. We show that the previously noted differences in NADP-MDH activity levels among C3, C4, and C3-C4 Flaveria species are in part due to interspecific differences in mRNA accumulation. We also show that the NADP-MDH gene appears to be present as a single copy among different Flaveria species, suggesting that a pre-existing gene has been reregulated during the evolution from C3 to C4 plants to accommodate the abundance and localization requirements of the C4 cycle.
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C4 isoform of NADP-malate dehydrogenase : cDNA cloning and expression in leaves of C4, C3, and C3-C4 intermediate species of Flaveria
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Autor/in / Beteiligte Person: | MCGONIGLE, B ; NELSON, T |
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Zeitschrift: | Plant physiology (Bethesda), Jg. 108 (1995), Heft 3, S. 1119-1126 |
Veröffentlichung: | Rockville, MD: American Society of Plant Physiologists, 1995 |
Medientyp: | academicJournal |
Umfang: | print, 1 p |
ISSN: | 0032-0889 (print) |
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