Coating polystyrene particles by adsorption of hydrophobically modified dextran
In: Langmuir, Jg. 11 (1995), Heft 7, S. 2344-2347
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Zugriff:
In order to decrease their hydrophobicity and thus to reduce the protein adsorption at their surface, polystyrene-divinylbenzene (PS-DVB) particles have been coated with dextran. To favor its adsorption, the polysaccharide was previously substituted with low concentrations of phenoxy groups. The amount of dextran adsorbed onto the PS-DVB surface was examined as a function of the polymer concentration, and various samples of beads, differing in surface dextran concentration, were prepared. The adsorbed polymeric layer was then chemically cross-linked and its stability was checked in the presence of sodium dodecyl sulfate. The hydrophilic character of the various resulting beads was evaluated toward the adsorption of bovine serum albumin, a strongly hydrophobic protein. By this study, the conditions for coating polystyrene particles were optimized and the dextran layer was shown to greatly reduce the nonspecific adsorption of proteins.
Titel: |
Coating polystyrene particles by adsorption of hydrophobically modified dextran
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Autor/in / Beteiligte Person: | FOURNIER, C ; LEONARD, M ; LE COQ-LEONARD, I ; DELLACHERIE, E |
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Zeitschrift: | Langmuir, Jg. 11 (1995), Heft 7, S. 2344-2347 |
Veröffentlichung: | Washington, DC: American Chemical Society, 1995 |
Medientyp: | academicJournal |
Umfang: | print, |
ISSN: | 0743-7463 (print) |
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