Growth and differentiation of normal human melanocytes in a TPA-free, cholera toxin-free, low-serum medium and influence of keratinocytes
In: Archives of dermatological research (Print), Jg. 285 (1993), Heft 7, S. 385-392
Online
academicJournal
- print, 23 ref
Zugriff:
Melanocyte cultures were obtained from a modification of the keratinocyte culture system MCDB153. Either promelanocytes or mature melanocytes were selected from epidermal cell primary cultures. Pure subcultures of actively dividing melanocytes of both types were grown in a low-serum medium totally deprived of TPA and cholera toxin called melanocyte growth medium (MGM). Early passaged cells from MGM primary cocultures were similar to normal adult human melanocytes in vivo, exhibiting numerous melanosomes, strong dopa positivity and a high dendricity. The ability of MGM to support melanocyte growth was mainly a consequence of its basic composition, combined with a low serum concentration.
Titel: |
Growth and differentiation of normal human melanocytes in a TPA-free, cholera toxin-free, low-serum medium and influence of keratinocytes
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Autor/in / Beteiligte Person: | DONATIEN, P ; SURLEVE-BAZEILLE, J. E ; THODY, A. J ; TAÏEB, A |
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Zeitschrift: | Archives of dermatological research (Print), Jg. 285 (1993), Heft 7, S. 385-392 |
Veröffentlichung: | Berlin: Springer, 1993 |
Medientyp: | academicJournal |
Umfang: | print, 23 ref |
ISSN: | 0340-3696 (print) |
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