Correction of the class H defect in glucosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone
In: The Journal of biological chemistry (Print), Jg. 268 (1993), Heft 28, S. 20733-20736
Online
academicJournal
- print, 40 ref
Zugriff:
Previous attempts to express glycosylphoaphatidylinositol-anchored proteins in Ltk- cells have not been successful because Ltk- cells cannot synthesize N-acetylglucoamine-phosphatidylinoaitol, the first intermediate in anchor biosynthesis. Using complementation cloning, we have identified a human cDNA that corrects the defect in anchor biosynthesis and allows the expression of glycosylphosphatidylinositol-anchored proteins in Ltk- cells. The nucleotide sequence predicts a novel cytosolic protein of 188 amino acids.
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Correction of the class H defect in glucosylphosphatidylinositol anchor biosynthesis in Ltk- cells by a human cDNA clone
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Autor/in / Beteiligte Person: | KAMITANI, T ; HUI-MING, CHANG ; ROLLINS, C ; WANECK, G. L ; YEH, E. T. H |
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Zeitschrift: | The Journal of biological chemistry (Print), Jg. 268 (1993), Heft 28, S. 20733-20736 |
Veröffentlichung: | Bethesda, MD: American Society for Biochemistry and Molecular Biology, 1993 |
Medientyp: | academicJournal |
Umfang: | print, 40 ref |
ISSN: | 0021-9258 (print) |
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