Effect of cryoprotective additives and cryopreservation protocol on sperm membrane lipid peroxidation and recovery of motile human sperm
In: Journal of andrology, Jg. 14 (1993), Heft 6, S. 472-478
Online
academicJournal
- print, 21 ref
Zugriff:
Sperm membrane damage during cryopreservation reduces the recovery of motile sperm. The present study investigates changes in sperm motility and membrane lipid peroxidation (LPO) in response to two changes in the standard sperm cryopreservation/thawing methodology: 1) the addition of platelet-activating factor (PAF) and pentoxifylline (PTX) as cryoprotective additives, and 2) the alteration of sample thawing time. PAF (1 μM) and PTX (3 mM) were added to fresh sperm samples prior to cryopreservation. After 2 weeks the samples were thawed either quickly (5 minutes at 37°C) or slowly (30 minutes at 4°C) and evaluated for sperm motility and LPO.
Titel: |
Effect of cryoprotective additives and cryopreservation protocol on sperm membrane lipid peroxidation and recovery of motile human sperm
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Autor/in / Beteiligte Person: | BELL, M ; RUN, WANG ; HELLSTROM, W. J. G ; SIKKA, S. C |
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Zeitschrift: | Journal of andrology, Jg. 14 (1993), Heft 6, S. 472-478 |
Veröffentlichung: | San Fransisco, CA: American Society of Andrology, 1993 |
Medientyp: | academicJournal |
Umfang: | print, 21 ref |
ISSN: | 0196-3635 (print) |
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