Overproduction of mannitol dehydrogenase in Rhodobacter sphaeroides
In: Applied microbiology and biotechnology, Jg. 41 (1994), Heft 5, S. 578-583
Online
academicJournal
- print, 21 ref
Zugriff:
Mannitol dehydrogenase (MDH; EC 1.1.1.67) from Rhodobacter sphaeroides Si4 was overproduced by constructing a strain that overexpresses the MDH gene and by producing high cell concentrations via fed-batch cultivation in a bioreactor. With the gene of mannitol dehydrogenase (mtlK) cloned into the expression vector pKK223-3 expression of MDH in Escherichia coli was obtained, but the specific enzyme activity was lower than in R. sphaeroides Si4. In order to overexpress mtlK in R. sphaeroides, plasmid pAK82 was constructed by cloning a DNA fragment carrying mtlK into the broad-host-range expression vector pRK415.
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Overproduction of mannitol dehydrogenase in Rhodobacter sphaeroides
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Autor/in / Beteiligte Person: | SCHNEIDER, K. H ; GIFFHORN, F |
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Zeitschrift: | Applied microbiology and biotechnology, Jg. 41 (1994), Heft 5, S. 578-583 |
Veröffentlichung: | Berlin: Springer, 1994 |
Medientyp: | academicJournal |
Umfang: | print, 21 ref |
ISSN: | 0175-7598 (print) |
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