Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway
In: EMBO journal (Print), Jg. 12 (1993), Heft 3, S. 869-877
Online
academicJournal
- print, 57 ref
Zugriff:
We have found that brefeldin A (BFA) inhibited the growth of an ise1 mutant of Saccharomyces cerevisiae. Genetic complementation and mapping studies demonstrated that ise1 was allelic to erg6, a gene required for the biosynthesis of the principal membrane sterol of yeast, ergosterol. Treatment of ise1 cells with BFA resulted in an immediate block in protein transport through the secretory pathway. Vacuolar carboxypeptidase Y (CPY) and the secreted pheromone α-factor accumulated as both the core glycosylated (ER) and α1,6 mannosylated (early Golgi) forms in drug-treated cells.
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Brefeldin A reversibly blocks early but not late protein transport steps in the yeast secretory pathway
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Autor/in / Beteiligte Person: | GRAHAM, T. R ; SCOTT, P. A ; EMR, S. D |
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Zeitschrift: | EMBO journal (Print), Jg. 12 (1993), Heft 3, S. 869-877 |
Veröffentlichung: | London: Nature Publishing Group, 1993 |
Medientyp: | academicJournal |
Umfang: | print, 57 ref |
ISSN: | 0261-4189 (print) |
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