Cytolytic effects and biochemical changes induced by extracellular ATP to isolated hepatocytes
In: Biochimica et biophysica acta. Molecular cell research, Jg. 1176 (1993), Heft 1-2, S. 1-6
academicJournal
- print, 31 ref
Zugriff:
Cell death, as estimated by the release of lactate dehydrogenasc (LDH), was induced by incubating isolated hepatocytes for 60 min in the presence of extracellular ATP (ecATP), while AMP, adenosine, GTP and UTP were without any significant effects, even when tested at 3 mM (final concentration). At such a concentration, the release of LDH induced by ecATP, but also by ecADP, reached almost 50% and 30%, respectively. Since UTP and GTP (which have no lytic effects) were able to activate phosphorylase a at the same rate as ATP, we excluded the possibility that an increase of free cytosolic Ca2+ triggers the onset of a process leading to cell lysis.
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Cytolytic effects and biochemical changes induced by extracellular ATP to isolated hepatocytes
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Autor/in / Beteiligte Person: | TINTON, S. A ; LEFEBVRE, V. H ; COUSIN, O. C ; BUC-CALDERON, P. M |
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Zeitschrift: | Biochimica et biophysica acta. Molecular cell research, Jg. 1176 (1993), Heft 1-2, S. 1-6 |
Veröffentlichung: | Amsterdam: Elsevier Science, 1993 |
Medientyp: | academicJournal |
Umfang: | print, 31 ref |
ISSN: | 0167-4889 (print) |
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