Effects of N-terminal truncations upon chloroplast NADP-malate dehydrogenases from pea and spinach
In: Biochimica et biophysica acta. Protein structure and molecular enzymology, Jg. 1163 (1993), Heft 1, S. 10-16
academicJournal
- print, 23 ref
Zugriff:
Using the purification procedure of Fickenscher and Scheibe (Biochim. Biophys. Acta 749 (1983), 249-254) and a modification of the method, we produced a series of NADP-MDH forms from spinach and pea-leaf extracts that were characterized by a stepwise shortening of the N-terminal sequences. Limited proteolysis of the enzymes resulted in the generation of even shorter forms. Immunoprecipitation of the NADP-MDH from crude extracts revealed that the sequences of the intact enzymes from pea, spinach and maize started at a position (Ser) identical with that established for the Sorghum enzyme (Cretin, C., et al. (1990) Eur. J. Biochem. 192, 299-303).
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Effects of N-terminal truncations upon chloroplast NADP-malate dehydrogenases from pea and spinach
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Autor/in / Beteiligte Person: | OCHERETINA, O ; HARNECKER, J ; ROTHER, T ; SCHMID, R ; SCHEIBE, R |
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Zeitschrift: | Biochimica et biophysica acta. Protein structure and molecular enzymology, Jg. 1163 (1993), Heft 1, S. 10-16 |
Veröffentlichung: | Amsterdam: Elsevier, 1993 |
Medientyp: | academicJournal |
Umfang: | print, 23 ref |
ISSN: | 0167-4838 (print) |
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