Lipopolysaccharide epitope specificity and binding cross reactivity of the human IgM anti-lipid a monoclonal antibody SdJ5-1.17.15
In: Molecular immunology, Jg. 30 (1993), Heft 10, S. 895-902
academicJournal
- print, 1 p. 1/2
Zugriff:
The binding properties and specificity of the SdJ5-1.17.15 (SdJ5) human IgM monoclonal antibody (mAb), prepared against S. minnesota R595 heat killed whole organisms, were assessed by dot blot assay in vitro. In that assay, lipopolysaccharide (LPS) related antigens, immobilized on nitrocellulose membrane, were reacted with the test and control human IgM antibodies. Results indicated the SdJ5 mAb reacted specifically with lipid A, a component of LPS, from a variety of bacterial species, but not with the whole LPS molecule. The inability of the mAb to react with whole LPS in dot blot assay was attributed to the possible effect of the solid phase on epitope exposure or structure.
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Lipopolysaccharide epitope specificity and binding cross reactivity of the human IgM anti-lipid a monoclonal antibody SdJ5-1.17.15
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Autor/in / Beteiligte Person: | MOHAMMAD, KAZEMI ; HUNTENBURG, C. C ; BUBBERS, J. E |
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Zeitschrift: | Molecular immunology, Jg. 30 (1993), Heft 10, S. 895-902 |
Veröffentlichung: | Oxford: Elsevier, 1993 |
Medientyp: | academicJournal |
Umfang: | print, 1 p. 1/2 |
ISSN: | 0161-5890 (print) |
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