Characteristics of [125I]ω-conotoxin MVIIA binding to rat neocortical membranes
In: Neuroscience letters, Jg. 161 (1993), Heft 1, S. 113-116
academicJournal
- print, 28 ref
Zugriff:
[125ω-Conotoxin MVIIA (ω-CTM) binding to N-type voltage-sensitive calcium channels (VSCCs) was characterized using rat neocortical membranes,[125I]ω-CTM bound rapidly and with high affinity; these parameters were similar to binding using ω-conotoxin GVIA ([125I]ω-CGT). Unlike [125I]ω-CTG, however, [125I]ω-CTM readily dissociated from its binding site. Monovalent and divalent cations, polyamines, and aminoglycosides inhibited [125I]ω-CTM binding. Since [125I]ω-CTM appears to bind to the same site as [125I]ω-CGT in mammalian neurons, the reversibility of [125I]ω-CTM binding makes this ligand preferable for equilibrium binding analyses.
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Characteristics of [125I]ω-conotoxin MVIIA binding to rat neocortical membranes
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Autor/in / Beteiligte Person: | STOEHR, S. J ; DOOLEY, D. J |
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Zeitschrift: | Neuroscience letters, Jg. 161 (1993), Heft 1, S. 113-116 |
Veröffentlichung: | Shannon: Elsevier, 1993 |
Medientyp: | academicJournal |
Umfang: | print, 28 ref |
ISSN: | 0304-3940 (print) |
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