Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene
In: Journal of bacteriology, Jg. 174 (1992), Heft 16, S. 5346-5353
Online
academicJournal
- print, 32 ref
Zugriff:
The gene (mdh) coding for methanol dehydrogenase (MDH) or thermotolerant, methylotroph Bacillus methanolicus C1 has been cloned and sequenced. The deduced amino acid sequence or the mdh gene exhibited similarity to those or five other alcohol dehydrogenase (type III) enzymes, which are distinct from the long-chain zinc-containing (type I) or short-chain zinc-lacking (type II) enzymes. Highly efficient expression or the mdh gene in Escherichia coli was probably driven from its own promoter sequence.
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Cloning, expression, and sequence analysis of the Bacillus methanolicus C1 methanol dehydrogenase gene
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Autor/in / Beteiligte Person: | DE VRIES, G. E ; ARFMAN, N ; TERPSTRA, P ; DIJKHUIZEN, L |
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Zeitschrift: | Journal of bacteriology, Jg. 174 (1992), Heft 16, S. 5346-5353 |
Veröffentlichung: | Washington, DC: American Society for Microbiology, 1992 |
Medientyp: | academicJournal |
Umfang: | print, 32 ref |
ISSN: | 0021-9193 (print) |
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