Three-Dimensional Human Cell Cultures for Cytotoxicity Testing of Dental Filling Materials
In: Acta Stomatologica Croatica, Jg. 48 (2014), Heft 2, S. 99-108
Online
academicJournal
Zugriff:
Objectives: So far, bovine immortalized pulp cells have been used as three dimensional cultures for cytotoxicity testing of filling materials in the dentin barrier test (DBT). In this study, the use of human pulp-derived cells was evaluated, which would better simulate the clinical situation, and a composite material with a new resin base was tested. Materials and Methods: SV40-transfected human pulp cells (tHPC) were cultured in hydrogels (fibrin, peptide, collagen) and mechanical properties and cell viability (MTT or WST-1) were determined. For cell cultures in collagen, a four week - proliferation assay was performed (WST-1). After 14 days of three-dimensional culture in collagen, tHPC were introduced into the DBT with 200 μm dentin disks. After a 24-hour incubation under perfusion (0.3 ml/h), the following materials were applied according to the manufacturers’ instructions (1) President (Coltene): negative (non-toxic) control, (2) CaGPG14 (ISO 7405): positive (toxic) control, (3) Tetric EvoCeram (Ivoclar Vivadent) with Clearfil SEBond (Kuraray, reference material), (4) N´Durance (Sepodont, test material), (5) N´Durance with Clearfil SEBond. Cell viability was determined after 24-hour incubation (WST-1). The percentage of relative viability was calculated (negative control=100%) and statistically analyzed (Kruskal-Wallis-test, p
Titel: |
Three-Dimensional Human Cell Cultures for Cytotoxicity Testing of Dental Filling Materials
|
---|---|
Autor/in / Beteiligte Person: | Gröppel, Franziska ; Hiller, Karl-Anton ; Galler, Kerstin M. ; Schmalz, Gottfried |
Link: | |
Zeitschrift: | Acta Stomatologica Croatica, Jg. 48 (2014), Heft 2, S. 99-108 |
Veröffentlichung: | University of Zagreb. School of Dental Medicine, 2014 |
Medientyp: | academicJournal |
ISSN: | 0001-7019 (print) ; 1846-0410 (print) |
Schlagwort: |
|
Sonstiges: |
|