Acoustic Cell Patterning in Hydrogel for Three-Dimensional Cell Network Formation
In: Micromachines, Jg. 12 (2020-12-01), Heft 1, S. 3-3
Online
academicJournal
Zugriff:
In the field of engineered organ and drug development, three-dimensional network-structured tissue has been a long-sought goal. This paper presents a direct hydrogel extrusion process exposed to an ultrasound standing wave that aligns fibroblast cells to form a network structure. The frequency-shifted (2 MHz to 4 MHz) ultrasound actuation of a 400-micrometer square-shaped glass capillary that was continuously perfused by fibroblast cells suspended in sodium alginate generated a hydrogel string, with the fibroblasts aligned in single or quadruple streams. In the transition from the one-cell stream to the four-cell streams, the aligned fibroblast cells were continuously interconnected in the form of a branch and a junction. The ultrasound-exposed fibroblast cells displayed over 95% viability up to day 10 in culture medium without any significant difference from the unexposed fibroblast cells. This acoustofluidic method will be further applied to create a vascularized network by replacing fibroblast cells with human umbilical vein endothelial cells.
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Acoustic Cell Patterning in Hydrogel for Three-Dimensional Cell Network Formation
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Autor/in / Beteiligte Person: | Koo, Kyo-in ; Lenshof, Andreas ; Le Thi Huong ; Laurell, Thomas |
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Zeitschrift: | Micromachines, Jg. 12 (2020-12-01), Heft 1, S. 3-3 |
Veröffentlichung: | MDPI AG, 2020 |
Medientyp: | academicJournal |
ISSN: | 2072-666X (print) |
DOI: | 10.3390/mi12010003 |
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