Evaluation of GBV-C / HVG viremia in HIV-infected women
In: Revista do Instituto de Medicina Tropical de São Paulo, Jg. 54 (2012), Heft 1, S. 31-35
Online
academicJournal
Zugriff:
The present study aimed at standardizing a real-time quantitative polymerase chain reaction assay to evaluate the presence of GBV-C/HGV RNA. A "TaqMan" assay using primers and probe derived from the 5¢ NCR region was developed and validated. Two hundred and fifty-three plasma samples from HIV-infected women were tested for GBV-C viremia and antibody against the envelope protein 2. GBV-C RNA was detected in 22.5% of the patients whereas the antibody was identified in 25.3% of the cohort. Detection of viral RNA and of antibodies was mutually exclusive. Viral loads showed a mean of 1,777 arbitrary units / mL, being 1.1 and 13,625 arbitrary units / mL respectively the lowest and highest values measured. We conclude that the real-time quantitative polymerase chain reaction method developed is appropriate for the investigation of GBV-C RNA since it was shown to be highly specific and sensitive, as well as requiring few steps, preventing contamination and providing additional information as to the relative viremia of carriers, a parameter that must be included in studies evaluating the co-factors influencing the clinical outcome of HIV/AIDS.
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Evaluation of GBV-C / HVG viremia in HIV-infected women
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Autor/in / Beteiligte Person: | Synara Araújo Silva ; Célia Lima Rodrigues ; Aléia Faustino Campos ; José Eduardo Levi |
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Zeitschrift: | Revista do Instituto de Medicina Tropical de São Paulo, Jg. 54 (2012), Heft 1, S. 31-35 |
Veröffentlichung: | Universidade de São Paulo (USP), 2012 |
Medientyp: | academicJournal |
ISSN: | 1678-9946 (print) ; 0036-4665 (print) |
DOI: | 10.1590/S0036-46652012000100006 |
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