DIDS Reduces Ischemia/Reperfusion-Induced Myocardial Injury in Rats
In: Cellular Physiology and Biochemistry, Jg. 35 (2015), Heft 2, S. 676-688
Online
academicJournal
Zugriff:
Background/Aims: Anion channels such as chloride channel are known to participate in the regulation of a wide variety of cellular processes including development, differentiation, proliferation, apoptosis and regeneration. This study was designed to examine the effect of the non-selective anion channel blocker 4,4'-Diisothiocyanostilbene-2, 2'-disulfonic acid (DIDS) on cardiac function and apoptosis using a rat model of ischemia/reperfusion (I/R). Methods: Fifty male SD rats were randomly divided into the following groups including sham, I/R and I/R+DIDS (7, 14 or 28 mg/kg). In DIDS group, rats received DIDS treatment (4 ml/kg/hr) at the beginning of reperfusion for 2 hrs using a programmed micro-pump. Cardiac function was evaluated including left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP) as well as positive and negative maximal derivatives of left ventricular pressure (± dP/dtmax). Myocardial infarct size was detected using the double staining with 2, 3, 5-triphenyl-2H-tetra-zolium chloride (TTC) and Evan's blue dye. DNA ladder, TUNEL assay, Bax and Bcl-2 protein levels were evaluated. Levels of ROS and Akt phosphorylation were detected. Results: I/R injury compromised cardiac function as manifested by reduced LVSP and ± dP/dtmax as well as pronounced apoptosis. I/R-induced cardiac anomalies were markedly ameliorated by DIDS. DIDS retarded I/R-induced myocardial infarct and apoptosis. In addition, DIDS ameliorated I/R-induced ROS production and Akt dephosphorylation in the heart. Conclusion: Taken together, our data revealed that DIDS may protect cardiomyocytes against I/R injury as evidenced by improved cardiac function, Bcl-2, Akt phosphorylation, and reduced myocardial apoptosis, Bax expression, ROS production and myocardial infarct size.
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DIDS Reduces Ischemia/Reperfusion-Induced Myocardial Injury in Rats
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Autor/in / Beteiligte Person: | Wang, Xiaoming ; Cao, Yanan ; Shen, Mingzhi ; Wang, Bo ; Zhang, Weiwei ; Liu, Yan ; He, Xiaole ; Wang, Lin ; Xia, Yuesheng ; Ding, Mingge ; Xu, Xihui ; Ren, Jun |
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Zeitschrift: | Cellular Physiology and Biochemistry, Jg. 35 (2015), Heft 2, S. 676-688 |
Veröffentlichung: | Cell Physiol Biochem Press GmbH & Co KG, 2015 |
Medientyp: | academicJournal |
ISSN: | 1015-8987 (print) ; 1421-9778 (print) |
DOI: | 10.1159/000369728 |
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