Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S]
In: Journal of Lipid Research, Jg. 54 (2013), Heft 3, S. 831-842
Online
academicJournal
Zugriff:
Very long-chain fatty acids (VLCFAs), fatty acids with chain-length greater than 20 carbons, possess a wide range of biological functions. However, their roles at the molecular level remain largely unknown. In the present study, we screened for multicopy suppressors that rescued temperature-sensitive growth of VLCFA-limited yeast cells, and we identified the VPS21 gene, encoding a Rab GTPase, as such a suppressor. When the vps21Δ mutation was introduced into a deletion mutant of the SUR4 gene, which encodes a VLCFA elongase, a synthetic growth defect was observed. Endosome-mediated vesicular trafficking pathways, including endocytosis and the carboxypeptidase Y (CPY) pathway, were severely impaired in sur4Δ vps21Δ double mutants, while the AP-3 pathway that bypasses the endosome was unaffected. In addition, the sur4Δ mutant also exhibited a synthetic growth defect when combined with the deletion of VPS3, which encodes a subunit of the class C core vacuole/endosome tethering (CORVET) complex that tethers transport vesicles to the late endosome/multivesicular body (MVB). These results suggest that, of all the intracellular trafficking pathways, requirement of VLCFAs is especially high in the endosomal pathways.
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Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids[S]
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Autor/in / Beteiligte Person: | Obara, Keisuke ; Kojima, Ryo ; Kihara, Akio |
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Zeitschrift: | Journal of Lipid Research, Jg. 54 (2013), Heft 3, S. 831-842 |
Veröffentlichung: | Elsevier, 2013 |
Medientyp: | academicJournal |
ISSN: | 0022-2275 (print) |
DOI: | 10.1194/jlr.M034678 |
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