Dissemination of Pseudomonas aeruginosa blaNDM-1-Positive ST308 Clone in Singapore
In: Microbiology Spectrum, Jg. 11 (2023-06-01), Heft 3
Online
academicJournal
Zugriff:
ABSTRACT Pseudomonas aeruginosa ST308 clone has been reported to carry carbapenemase genes such as blaIMP and blaVIM but has been rarely associated with blaNDM-1. A total of 199 P. aeruginosa ST308 clinical and environmental isolates obtained between April 2019 and November 2020 from a tertiary-care hospital in Singapore were characterized using whole-genome sequencing. In addition, 71 blaNDM-1-positive ST308 whole-genome sequences from two other local tertiary-care hospitals in Singapore and 83 global blaNDM-1-negative ST308 whole-genome sequences in public databases were included to assess phylogenetic relationships and perform genome analyses. Phylogenetic analysis and divergent time estimation revealed that blaNDM-1-positive P. aeruginosa ST308 was introduced into Singapore in 2005 (95 % highest posterior density: 2001 to 2008). Core genome, resistome, and analyses of all local blaNDM-1-positive ST308 isolates showed chromosomal integration of multiple antibiotic resistance genes (ARGs) [aac(3)-Id, aac(6′)-Il, aadA6, aadA11, dfrB5, msr(E), floR, sul2, and qnrVC1], which was absent in global blaNDM-1-negative ST308 sequences. Most ARGs and virulence genes were conserved across isolates originating from the three different local hospitals. Close genetic relatedness of the blaNDM-1-positive ST308 clinical and environmental isolates suggests cocirculation between the hospital environment and human hosts with the hospital environment as a potential reservoir. Core genome single nucleotide polymorphism analyses revealed possible clonal transmission of blaNDM-1-positive ST308 isolates between the three hospitals over 7 years. Bloodstream isolates accounted for six of 95 (6.3%) clinical isolates. This study reports the introduction of a pathogenic blaNDM-1-positive P. aeruginosa ST308 more than a decade ago in Singapore and warrants surveillance for wider dissemination. IMPORTANCE P. aeruginosa is a Gram-negative opportunistic pathogen ubiquitously found in the environment and a major cause of nosocomial infections. While the P. aeruginosa ST308 clone has been known to bear blaIMP and blaVIM among global isolates, reports of blaNDM-1-positive P. aeruginosa ST308 are rare. The local blaNDM-1-positive P. aeruginosa ST308 isolates detected in this study appear to be unique to this region, with evidence of chromosomal acquisition of multiple ARGs compared to global blaNDM-1-negative P. aeruginosa ST308 isolates. Surveillance in Singapore and beyond for dissemination is essential to determine whether existing measures are sufficient to control the spread of this ST308 clone.
Titel: |
Dissemination of Pseudomonas aeruginosa blaNDM-1-Positive ST308 Clone in Singapore
|
---|---|
Autor/in / Beteiligte Person: | Sai Rama Sridatta Prakki ; Pei Yun Hon ; Ze Qin Lim ; Natascha May Thevasagayam ; Song Qi Dennis Loy ; Partha Pratim De ; Marimuthu, Kalisvar ; Vasoo, Shawn ; Oon Tek Ng |
Link: | |
Zeitschrift: | Microbiology Spectrum, Jg. 11 (2023-06-01), Heft 3 |
Veröffentlichung: | American Society for Microbiology, 2023 |
Medientyp: | academicJournal |
ISSN: | 2165-0497 (print) |
DOI: | 10.1128/spectrum.04033-22 |
Schlagwort: |
|
Sonstiges: |
|