Antioxidant and Nephroprotective Activities of Aconitum heterophyllum Root in Glycerol Induced Acute Renal Failure in Rats
In: Journal of Clinical and Diagnostic Research, Jg. 10 (2016), Heft 3, S. FF01-FF05
Online
academicJournal
Zugriff:
Aim: The present study was to evaluate the antioxidant and nephroprotective activities of ethanolic extract of Aconitum heterophyllum root (EEAHR) in glycerol induced acute renal failure (ARF) in Wistar albino rats. Materials and Methods: In vitro antioxidant activity of EEAHR was assessed using the 2, 2-diphenyl-picrylhydrazyl (DPPH assay), nitric oxide radical scavenging (NO assay), hydrogen peroxide (H2 O2 assay) and ferric reducing antioxidant power (FRAP) scavenging activity assays. In vivo study, rats were divided into four groups of six each for assessing the nephroprotective activity. Group-1 received normal saline, group-2 received 50% glycerol (10 ml/kg) alone, group-3 received glycerol and 250 mg/ kg of EEAHR and group-4 received glycerol and 500 mg/kg of EEAHR. The renal injury and recovery was measured by serum creatinine, blood urea nitrogen (BUN), total proteins, albumin, urine output and histopathological changes. Results: In vitro antioxidant activity of root extract was found to be equal to Vitamin C and in an in vivo study root extract treated animals showed significant attenuation of biochemical parameters and histopathological changes of the kidney compared to glycerol treated group and it was found to be more significant with the extract at 500 mg/kg than 250mg/kg. Conclusion: The present study revealed that Aconitum heterophyllum root has shown antioxidant and nephroprotective activities.
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Antioxidant and Nephroprotective Activities of Aconitum heterophyllum Root in Glycerol Induced Acute Renal Failure in Rats
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Autor/in / Beteiligte Person: | Venu Gopala Rao Konda ; Eerike, Madhavi ; Lakshmipathy Prabhu Raghuraman ; Maignana Kumar Rajamanickam |
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Zeitschrift: | Journal of Clinical and Diagnostic Research, Jg. 10 (2016), Heft 3, S. FF01-FF05 |
Veröffentlichung: | JCDR Research and Publications Private Limited, 2016 |
Medientyp: | academicJournal |
ISSN: | 2249-782X (print) ; 0973-709X (print) |
DOI: | 10.7860/JCDR/2016/10798.7388 |
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