Reprogramming of human peripheral blood mononuclear cells from a patient suffering from recurrent hydatidiform mole to an iPSC line FAHZUi001-A carrying a homozygous p.Gln421Ter mutation in NLRP7 gene
In: Stem Cell Research, Jg. 53 (2021-05-01), Heft 102361-
Online
academicJournal
Zugriff:
Recurrent hydatidiform mole (RHM) is characterized by the occurrence of at least twice hydatidiform mole. Unlike sporadic complete hydatidiform moles (CHMs), which are androgenetic with 2 paternal chromosomes, CHMs associated with familial recurrence are genetically biparental with a maternal and a paternal chromosome. NLRP7 mutations have been reported in 55% of RHM cases. Here, we generated induced pluripotent stem cells (iPSCs) from a patent with NLRP7 gene mutation c.1261C > T by reprogramming peripheral blood mononuclear cells by non-integrated method. The resulting iPSCs carrying NLRP7 mutation, had normal karyotype, expressed pluripotency markers, and could differentiate into three germ layers in vivo.
Titel: |
Reprogramming of human peripheral blood mononuclear cells from a patient suffering from recurrent hydatidiform mole to an iPSC line FAHZUi001-A carrying a homozygous p.Gln421Ter mutation in NLRP7 gene
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Autor/in / Beteiligte Person: | Gong, Tingyu ; Wang, Hongkun ; Huang, Bo ; Wang, Hao ; Zhang, Peiwen ; Qian, Jianhua |
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Zeitschrift: | Stem Cell Research, Jg. 53 (2021-05-01), Heft 102361- |
Veröffentlichung: | Elsevier, 2021 |
Medientyp: | academicJournal |
ISSN: | 1873-5061 (print) |
DOI: | 10.1016/j.scr.2021.102361 |
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