Kajian In vitro Aktivitas Sel-Sel Trofoblas Blastosis Mencit Aging dan Pengaruhnya terhadap Kegagalan Implantasi
In: Jurnal Veteriner, Jg. 10 (2009-03-01), Heft 1
Online
academicJournal
Zugriff:
The objectives of this in vitro study were to investigate the hatching rate, the outgrowth diameter andthe activity of mitochondria Nicotiamide Adenin Dinucleotide Dyhidrogenage (NADH)-CoQ reductase ofblastocysts trophoblast cells from aging mice. Blastocysts of aging (age >12 months) and young productive(age 2 months) mice were collected from the cornua utery at day-4 of pregnancy and were cultured inmDMEM medium supplemented with 10% New Born Calf Serum (NBCS), 10% ITS, and 50 ?g/mlgentamicine, in 5% CO2 incubator at 37°C for 10 days. The blastocysts hatching rate and the trophoblastsmonolayer were examined for their diameter outgrowth and the NADH-CoQ reductase activity. The resultsshowed that the hatching rate, the trophoblast outgrowth diameter and the activity of NADH-CoQ reductaseof blastocysts collected from productive mice were significantly higher than those collected from the agingmice (P
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Kajian In vitro Aktivitas Sel-Sel Trofoblas Blastosis Mencit Aging dan Pengaruhnya terhadap Kegagalan Implantasi
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Autor/in / Beteiligte Person: | Djuwita, Ita ; Helmita, Roza ; Winarto, Adi ; Wahyudin |
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Zeitschrift: | Jurnal Veteriner, Jg. 10 (2009-03-01), Heft 1 |
Veröffentlichung: | Universitas Udayana, 2009 |
Medientyp: | academicJournal |
ISSN: | 1411-8327 (print) ; 2477-5665 (print) |
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