Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas
In: Scientific Reports, Jg. 14 (2024), Heft 1, S. 1-15
Online
academicJournal
Zugriff:
Abstract Exocrine and endocrine pancreas are interconnected anatomically and functionally, with vasculature facilitating bidirectional communication. Our understanding of this network remains limited, largely due to two-dimensional histology and missing combination with three-dimensional imaging. In this study, a multiscale 3D-imaging process was used to analyze a porcine pancreas. Clinical computed tomography, digital volume tomography, micro-computed tomography and Synchrotron-based propagation-based imaging were applied consecutively. Fields of view correlated inversely with attainable resolution from a whole organism level down to capillary structures with a voxel edge length of 2.0 µm. Segmented vascular networks from 3D-imaging data were correlated with tissue sections stained by immunohistochemistry and revealed highly vascularized regions to be intra-islet capillaries of islets of Langerhans. Generated 3D-datasets allowed for three-dimensional qualitative and quantitative organ and vessel structure analysis. Beyond this study, the method shows potential for application across a wide range of patho-morphology analyses and might possibly provide microstructural blueprints for biotissue engineering.
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Multiscale and multimodal imaging for three-dimensional vascular and histomorphological organ structure analysis of the pancreas
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Autor/in / Beteiligte Person: | Gabriel Alexander Salg ; Steinle, Verena ; Labode, Jonas ; Wagner, Willi ; Studier-Fischer, Alexander ; Reiser, Johanna ; Farjallah, Elyes ; Guettlein, Michelle ; Albers, Jonas ; Hilgenfeld, Tim ; Giese, Nathalia A. ; Stiller, Wolfram ; Nickel, Felix ; Loos, Martin ; Michalski, Christoph W. ; Kauczor, Hans-Ulrich ; Hackert, Thilo ; Dullin, Christian ; Mayer, Philipp ; Hannes Goetz Kenngott |
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Zeitschrift: | Scientific Reports, Jg. 14 (2024), Heft 1, S. 1-15 |
Veröffentlichung: | Nature Portfolio, 2024 |
Medientyp: | academicJournal |
ISSN: | 2045-2322 (print) |
DOI: | 10.1038/s41598-024-60254-9 |
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