Studies on the Structure and Production of Bacillus macerans Cyclodextrin Glucanotransferase
2000
Hochschulschrift
Zugriff:
88
The study was aimed to investigate the structure and functional relationships the mutant Bacillus macerans cyclodextrin glucanotransferases (CGTase) generated by mutagenesis. And the investigate production of cyclodextrin (CD) with B. subtilis (pHG) CGTase. The plasmid - pUG containing the cloned B. macerans cgt gene was mutated with random mutagenesis. Mutated plasmids were transformed into E. coli. Seven mutant strains were selected on solid screening plates from 20,000 clones, and DNA sequencing identified mutations of the cgt gene. Among the seven strains, five showed one-amino-acid substitution, and two showed two-amino-acid alteration. All mutant CGTases still had the activities for cyclization and coupling reactions. The relative specific activities for either reaction differed with the mutants(p
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Studies on the Structure and Production of Bacillus macerans Cyclodextrin Glucanotransferase
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Autor/in / Beteiligte Person: | Chen, Kaui-Hwang ; 陳貴凰 |
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Veröffentlichung: | 2000 |
Medientyp: | Hochschulschrift |
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