Induction of class I low moleculer mass heat shock protein genes and a pathogenesis-related gene in rice by biotic and abiotic stresses
2003
Hochschulschrift
Zugriff:
91
HSPs are produced in microorganisms, plants and animals to protect tissues from high temperature stress. The functions of HSPs were reported in many living organisms. Low molecular mass HSPs (LMM HSPs, or small HSPs, smHSPs) are especially abundant in plants and play a major role in thermoprotection. On the other hand, pathogenesis-related (PR) proteins are associated with systemic acquired resistance (SAR) in plant defense responses. Overexpressing the PR genes may protect transgenic plants from pathogen attacks. A number of genes, including THE HSP70 family, were induced in soybean upon infection with cyst nematode. In animal system, HSPs are also related with inflammation but no report has been presented to connect HSPs with pathogen resistance in plants. The objective of this study was to investigate the expression of class I LMM HSP genes and PR-1 gene under biotic and abiotic stresses in rice seedlings and plants. The class I LMM HSP genes and PR-1 gene expression in rice seedlings treated with 41°C, salicylic acid, or wounding, and in rice plants inoculated with Rhizoctonia solani R96 was observed in this study. Among the six rice class I LMM HSP genes tested, the Oshsp18.0 expression was induced by 4 h of wounding treatment to a level of about 1.62-fold higher than that by control treatment, similar to that of 1.8-fold by 41°C HS treatment, according to the results of reverse transcription (RT)-polymerase chain reaction (PCR). The results of western blot analysis using polyclonal antibodies specific to the class I LMM HSPs also suggested that 4 h of salicylic acid treatment induced the accumulation of class I LMM HSPs. However, no much expression of the rice PR-1 gene was induced by the above-mentioned abiotic treatments as suggested by the results of RT-PCR and northern hybridization. On the other hand, PR-1 gene expression was induced, and related to pathogenesis by Rhizoctonia solani R96 infection. Four class I LMM HSP genes Oshsp16.9B, Oshsp16.9C, and Oshsp17.0 were induced by Rhizoctonia solani R96 infection. The results here suggested that, beside the PR proteins, the HSPs may also play a role in plant defense mechanism against the pathogen attack.
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Induction of class I low moleculer mass heat shock protein genes and a pathogenesis-related gene in rice by biotic and abiotic stresses
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Autor/in / Beteiligte Person: | Hsi-Sming, Chang ; 張喜民 |
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Veröffentlichung: | 2003 |
Medientyp: | Hochschulschrift |
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