Protective and Immunomodulatory effect of Gin-Gyo-San in a murine model of acute lung inflammation
2007
Hochschulschrift
Zugriff:
95
To investigate the effects of Gingyo-san (GGS), traditional Chinese medicinal formula, on the acute lung inflammation induced by LPS in vivo. Mice were challenged with intratracheal LPS before with treatment GGS or vehicle. In Lung morphology, GGS is better than Xia-Bai-San (XBS) and Hesperidin that reducing the infiltration of activated polymorphonuclear neutrophils in the airways, decreasing pulmonary edema, reduced nitrosative stress, and improved lung morphology. ELISA or RT-PCR detected the expression of cytokines in BALF and lung tissue. The mechanism of these benefits by treatment GGS including attenuating expression TNFα, IL-1β, IL-6, KC, MCP-1, MIP-2, iNOS, and activation of nuclear factor (NF-κB and AP-1) in BALF and lung tissue. Particularly, GGS also enhanced the anti-inflammatory cytokine (IL-10) and limited the acute lung inflammation. In vitro, HES suppressed the expression of IL-8 on A549 cells and THP-1 cells, the expression of TNFα, IL-1β, and IL-6 on THP-1 cells, the expression of ICAM-1 and VCAM-1 on A549 cells which effect cell adhesion function. The suppression of those molecules is controlled by NF-κB and AP-1, which are activated by IκB and MAPK pathways. We also demonstract that GGS, Flos Lonicera, and chlorogenic acid induce the IL-10 secretion in LPS-induced RAW 264.7 cell lines. Therefore, GGS protection activity against LPS-induced lung inflammatory mediators release and might be beneficial in the treatment of endotoxin-associated inflammation.
Titel: |
Protective and Immunomodulatory effect of Gin-Gyo-San in a murine model of acute lung inflammation
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Autor/in / Beteiligte Person: | Yeh, Chia-Chou ; 葉家舟 |
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Veröffentlichung: | 2007 |
Medientyp: | Hochschulschrift |
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